文献类型： 外文期刊

作者： You, Li ¹ ; Wang, Qian ³ ; Zhang, Tianxue ⁴ ; Xiao, Hongwei ⁵ ; Lv, Mengjiao ⁶ ; Lv, Hong ² ; Deng, Li ⁸ ; Zhang, Xuyao ⁹ ; Zhang, Yu ¹ ;

作者机构： 1.Fudan Univ, Shanghai Canc Ctr, Dept Gastr Surg, Shanghai 200032, Peoples R China

2.Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai 200032, Peoples R China

3.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Dept Obstet & Gynecol, Shanghai 200080, Peoples R China

4.Shanghai Open Univ, Yangpu Branch Campus, Shanghai 200082, Peoples R China

5.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Anim Embryo Engn & Mol Breeding Hubei Prov, Wuhan 430064, Peoples R China

6.Tongji Univ, Shanghai East Hosp, Dept Infect Dis, Shanghai 200120, Peoples R China

7.Fudan Univ, Shanghai Canc Ctr, Dept Pathol, Shanghai 200032, Peoples R China

8.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Gen Surg, Shanghai 200032, Peoples R China

9.Fudan Univ, Sch Pharm, Dept Biol Med, Shanghai 201203, Peoples R China

10.Fudan Univ, Shanghai Engn Res Ctr Immunotherapeut, Sch Pharm, Shanghai 201203, Peoples R China

期刊名称：ONCOGENE （ 影响因子：7.3； 五年影响因子：7.4 ）

ISSN： 0950-9232

年卷期： 2025 年 44 卷 28 期

页码：

收录情况： SCI

摘要： Resistance to anti-PD-1 therapy remains a significant challenge in gastric cancer (GC) treatment. Here, we revealed that the USP14-IMP2-CXCL2 axis in tumor-associated macrophages (TAMs) drove resistance by recruiting myeloid-derived suppressor cells (MDSCs). Endoscopic biopsy samples were obtained from patients with inoperable GC who were candidates for anti-PD-1 therapy. Single-cell RNA sequencing (scRNA-seq) analysis showed a higher prevalence of USP14+ TAMs in therapy-resistant cases, where USP14 was linked to the immunosuppressive phenotype of TAMs. Clinically, GC samples with elevated USP14+ TAM infiltration exhibited decreased CD8+ T cell presence and increased MDSC infiltration. In vivo experiments further confirmed that USP14+ TAMs facilitated resistance to anti-PD-1 therapy in GC, reduced the infiltration of CD8+ T cells, and significantly increased the infiltration of MDSCs. In particular, USP14+ TAMs markedly enhanced the recruitment of MDSCs into the GC microenvironment through the secretion of CXCL2. Mechanistically, USP14 stabilized the m6A reader IMP2 through deubiquitination, thus enhancing CXCL2 expression and secretion. Conversely, the E3 ligase RNF40 facilitated IMP2 degradation via increasing its ubiquitination, with USP14 and RNF40 dynamically balancing IMP2's protein abundance. Furthermore, animal experiments demonstrated that targeted intervention of USP14 markedly enhanced the sensitivity of GC to anti-PD-1 therapy. This study provided a comprehensive exploration of USP14's oncogenic roles in TAMs, suggesting a novel strategy to enhance the efficacy of anti-PD-1 therapy by inhibiting the USP14/IMP2/CXCL2 signaling axis in GC.The sc-RNA analysis revealed that infiltration of USP14+ TAM was significantly higher in anti-PD-1 resistant GC compared to anti-PD-1 sensitive GC (top). USP14 stabilized IMP2 protein in tumor-associated macrophages (middle-left), leading to recruitment of MDSCs into GC microenvironment through secretion of CXCL2 (middle-right), thus reducing the infiltration of CD8+ T cells and facilitating the resistance to anti-PD-1 therapy in GC (bottom). Comment: Dear editors, in formally published articles, figure legends could be placed beneath their corresponding figures. In this version, is this arrangement adopted for the sake of convenience during the proofreading process? If so, we understand and accept this layout.