A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of Takifugu rubripes
文献类型： 外文期刊
作者： Zhou, He 1 ; Sun, Yuqing 1 ; Li, Xin 1 ; Zhou, Ziyu 1 ; Ma, Kexin 1 ; Guo, Wenxuan 1 ; Liang, Yuting 1 ; Xie, Xingyi 1 ; Zhang, Jingxian 1 ; Wang, Qian 3 ; Liu, Yang 1 ;
作者机构： 1.Dalian Ocean Univ, Key Lab Mariculture, Agr Minist, PRC, Dalian 116023, Peoples R China
2.Dalian Ocean Univ, Key Lab Marine Bioresources Sustainable Utilizat, Dalian 116023, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
关键词： transcriptomic; low-temperature; pseudo-male; qPCR; Takifugu rubripes
期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）
ISSN： 2076-2615
年卷期： 2021 年 11 卷 12 期
页码：
收录情况： SCI
摘要： Simple Summary Our study analyzed the differentiation of transcriptomes in normal and sex-reverse Takifugu rubripes, and screened out 13 differentially expressed genes related to sex differentiation. This is the first report on the gonadal transcriptome of pseudo-males in Takifugu rubripes. Our results provide an important contribution to the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment. The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of Takifugu rubripes, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of T. rubripes (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (LOC101066759, dgat1, limk1, fbxl3, col6a3, fgfr3, dusp22b, svil, abhd17b, srgap3, tmem88b, bud4, and mustn10) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for T. rubripes and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.
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