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Overexpression of MxbHLH18 Increased Iron and High Salinity Stress Tolerance in Arabidopsis thaliana

文献类型： 外文期刊

作者： Liang, Xiaoqi 1 ; Li, Yingmei 1 ; Yao, Anqi 1 ; Liu, Wanda 2 ; Yang, Tianyu 1 ; Zhao, Mengfei 1 ; Zhang, Bingxiu 1 ; Han, Deguo 1 ;

作者机构： 1.Northeast Agr Univ, Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops North, Natl Local Joint Engn Res Ctr Dev & Utilizat Smal, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Hort Branch, Harbin 150040, Peoples R China

关键词： Malus xiaojinensis; MxbHLH18; salt stress; Fe stress; gene transformation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 14 期

页码：

收录情况： SCI

摘要： In the life cycle of apple, it will suffer a variety of abiotic stresses, such as iron stress and salt stress. bHLH transcription factors (TFs) play an indispensable role in the response of plants to stress. In this study, a new bHLH gene named MxbHLH18 was separated from Malus xiaojinensis. According to the results of subcellular localization, MxbHLH18 was localized in the nucleus. Salt stress and iron stress affected the expression of MxbHLH18 in Malus xiaojinensis seedlings to a large extent. Due to the introduction of MxbHLH18, the resistance of Arabidopsis thaliana to salt, high iron and low iron was significantly enhanced. Under the environmental conditions of high iron and low iron, the overexpression of MxbHLH18 increased many physiological indexes of transgenic Arabidopsis compared to wild type (WT), such as root length, fresh weight and iron content. The high level expression of MxbHLH18 in transformed Arabidopsis thaliana can not only increased the content of chlorophyll and proline, as well as increasing the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT); it also reduced the content of malondialdehyde (MDA), which was more obvious under high salt conditions. In addition, the relative conductivity, H2O2 content and O2- content in transgenic Arabidopsis decreased under salt stress. Meanwhile, MxbHLH18 can also regulate the expression of downstream genes associated with salt stress (AtCBF1/2/3, AtKIN1 and AtCOR15a/b) and iron stress (AtIRT1, AtFRO2, AtNAS2, ATACT2, AtZIF1 and AtOPT3). Therefore, MxbHLH18 can actively promote the adaptability of plants to the growth environment of salt and low and/or iron.

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