Characterisation and expression of a novel holotype crystal protein gene, cry56Aa1, from Bacillus thuringiensis strain Ywc2-8
文献类型： 外文期刊
作者： Zhu, J. 1 ; Zheng, A. P. 1 ; Tan, F. R. 1 ; Wang, S. Q. 1 ; Deng, Q. M. 1 ; Li, S. C. 1 ; Wang, L. X. 1 ; Li, P. 1 ;
作者机构： 1.Sichuan Agr Univ, Rice Res Inst, Wenjiang 611130, Sichuan, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China
关键词： holotype
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Bacillus thuringiensis isolate Ywc2-8, from soil in Sichuan Basin in western China, contains a spherical crystal harbouring two insecticidal crystal proteins with masses of 70 kDa and 130 kDa. A novel cry-type gene, encoding a 664 amino acid protein with 34% homology to cry29Aa1, was found and cloned from this strain. This gene belongs to a novel holotype cry and is designated as cry56Aa1. It was expressed in E. coli. Insecticidal activity assays showed that recombinant Cry56Aa1 was toxic to both Dipteran (Aedes aegypti) and Lepidopteran (Plutella xylostella and Helicoverpa armigera) pests. Cloning of this gene may help to overcome the increasing resistance of pests to currently used insecticides.
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