文献类型： 外文期刊

作者： Xu, Zhenbo ¹ ; Li, Yaqin ¹ ; Xu, Aijuan ³ ; Xue, Liang ¹ ; Soteyome, Thanapop ⁶ ; Yuan, Lei ⁷ ; Ma, Qin ⁸ ; Seneviratne, Gamini ⁹ ; Hong, Wei ¹⁰ ; Mao, Yuzhu ¹¹ ; Kjellerup, Birthe V. ¹¹ ; Liu, Junyan ¹² ;

作者机构： 1.South China Univ Technol, Engn Res Ctr Starch & Vegetable Prot Proc, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Sch Food Sci & Engn,Minist Educ, Guangzhou, Peoples R China

2.Shantou Univ, Dept Lab Med, Affiliated Hosp 2, Med Coll, Shantou, Guangdong, Peoples R China

3.Guangzhou Hybribio Med Lab, Guangzhou, Peoples R China

4.Guangdong Acad Sci, Inst Microbiol, Guangdong Prov Key Lab Microbial Safety & Hlth, State Key Lab Appl Microbiol Southern China, Guangzhou, Guangdong, Peoples R China

5.Guangdong Acad Sci, Key Lab Agr Microbi & Precis Applicat, Minist Agr & Rural Affairs, Guangzhou, Guangdong, Peoples R China

6.Rajamangala Univ Technol Phra Nakhon, Home Econ Technol, Bangkok, Thailand

7.Yangzhou Univ, Sch Food Sci & Engn, Yangzhou, Jiangsu, Peoples R China

8.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Key Lab Funct Foods,Guangdong Key Lab Agri Prod Pr, Minist Agr, Guangzhou, Peoples R China

9.Natl Inst Fundamental Studies, Kandy, Sri Lanka

10.Guangzhou Med Univ, GMU GIBH Joint Sch Life Sci, Guangzhou, Guangdong, Peoples R China

11.Univ Maryland, Dept Civil & Environm Engn, College Pk, MD USA

12.Zhongkai Univ Agr & Engn, Acad Contemporary Agr Engn Innovat, Coll Light Ind & Food Sci, Guangdong Prov Key Lab Lingnan Specialty Food Sci, Guangzhou, Peoples R China

13.Minist Agr, Key Lab Green Proc & Intelligent Mfg Lingnan Speci, Guangzhou, Peoples R China

关键词： Lactiplantibacillus plantarum subsp. plantarum; Candida albicans; polymicrobial transcriptomics; quorum-sensing system; pathogenesis and virulence determinants

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Candida albicans (C. albicans) and Lactiplantibacillus plantarum subsp. plantarum (L. plantarum) are frequently identified in various niches, but their dual-species interaction, especially with C. albicans in yeast form, remains unclear. This study aimed to investigate the dual-species interaction of L. plantarum and C. albicans, including proliferation, morphology, and transcriptomes examined by selective agar plate counting, microscopy, and polymicrobial RNA-seq, respectively. Maintaining a stable and unchanged growth rate, L. plantarum inhibited C. albicans yeast cell proliferation but not hyphal growth. Combining optical microscopy and atomic force microscopy, cell-to-cell direct contact and co-aggregation with L. plantarum cells surrounding C. albicans yeast cells were observed during dual-species interaction. Reduced C. albicans yeast cell proliferation in mixed culture was partially due to L. plantarum cell-free culture supernatant but not the acidic environment. Upon polymicrobial transcriptomics analysis, interesting changes were identified in both L. plantarum and C. albicans gene expression. First, two L. plantarum quorum-sensing systems showed contrary changes, with the activation of lamBDCA and repression of luxS. Second, the upregulation of stress response-related genes and downregulation of cell cycle, cell survival, and cell integrity-related pathways were identified in C. albicans, possibly connected to the stress posed by L. plantarum and the reduced yeast cell proliferation. Third, a large scale of pathogenesis and virulence factors were downregulated in C. albicans, indicating the potential interruption of pathogenic activities by L. plantarum. Fourth, partial metabolism and transport pathways were changed in L. plantarum and C. albicans. The information in this study might aid in understanding the behavior of L. plantarum and C. albicans in dual-species interaction. IMPORTANCE The anti-Candida albicans activity of Lactiplantibacillus plantarum has been explored in the past decades. However, the importance of C. albicans yeast form and the effect of C. albicans on L. plantarum had also been omitted. In this study, the dual-species interaction of L. plantarum and C. albicans was investigated with a focus on the transcriptomes. Cell-to-cell direct contact and co-aggregation with L. plantarum cells surrounding C. albicans yeast cells were observed. Upon polymicrobial transcriptomics analysis, interesting changes were identified, including contrary changes in two L. plantarum quorum-sensing systems and reduced cell survival-related pathways and pathogenesis determinants in C. albicans.