The survival and enterotoxin gene expression of Staphylococcus aureus planktonic and biofilm cells in quick-frozen food
文献类型： 外文期刊
作者： Liu, Junyan 1 ; Huang, Tengyi 3 ; Soteyome, Thanapop 4 ; Mao, Yuzhu 5 ; Luo, Yuting 5 ; Yuan, Lei 6 ; Ma, Qin 7 ; Liu, Gongliang 1 ; Qu, Chunyun 1 ; Seneviratne, Gamini 8 ; Kjellerup, Birthe, V 9 ; Xu, Zhenbo 3 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Acad Contemporary Agr Engn Innovat, Coll Light Ind & Food Sci, Guangdong Prov Key Lab Lingnan Specialty Food Sci, Guangzhou 510225, Peoples R China
2.Minist Agr, Key Lab Green Proc & Intelligent Mfg Lingnan Speci, Guangzhou 510225, Peoples R China
3.Shantou Univ, Dept Lab Med, Affiliated Hosp 2, Med Coll, Shantou, Guangdong, Peoples R China
4.Rajamangala Univ Technol Phra Nakhon, Home Econ Technol, Bangkok, Thailand
5.South China Univ Technol, Engn Res Ctr Starch & Vegetable Prot Proc, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Sch Food Sci & Engn,Minist Educ, Guangzhou 510640, Peoples R China
6.Yangzhou Univ, Sch Food Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China
7.Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Key Lab Funct Foods, Minist Agr,Sericultural & Agrifood Res Inst, Guangzhou 510610, Peoples R China
8.Natl Inst Fundamental Studies, Hantana Rd, Kandy, Sri Lanka
9.Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA
关键词： Rice and flour products; Staphylococcus aureus; VBNC; Enterotoxin B (SEB); Low temperature
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）
ISSN： 0023-6438
年卷期： 2023 年 187 卷
页码：
收录情况： SCI
摘要： Food safety issues caused by food-borne pathogenic microorganisms during low-temperature food storage have attracted broad attention. In the current study, Staphylococcus aureus cells in planktonic and different stages of biofilm formation were inoculated into four types of quick-frozen rice and flour products. The changes in cul-turable cell number of S. aureus in the artificially contaminated food samples stored at 4 degrees C and-20 degrees C were recorded for 60 days. Next, the PMA-qPCR method was used to explore the viable cell number and determine whether low temperature induced S. aureus enter into the viable but non-culturable (VBNC) state. Finally, the expression changes of enterotoxin gene seb in S. aureus were determined by RT-qPCR during food sample storage. Results showed that the culturable cell number of S. aureus was not significantly changed during 60 days except for the decreased culturable cell number in the 8 h biofilm cells in MF stored at-20 degrees C. In addition, S. aureus cells did not enter into the VBNC state during low temperature storage. Moreover, the seb gene was continuously expressed in most samples although with difference in expression level. The findings provided an alert for the risk of S. aureus contamination in quick-frozen rice and flour products.
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