文献类型： 外文期刊

作者： Ma, Jianhui ¹ ; Geng, Yuke ² ; Liu, Hong ¹ ; Zhang, Mengqi ¹ ; Liu, Shujuan ² ; Hao, Chenyang ² ; Hou, Jian ² ; Zhang, Youfu ² ; Zhang, Daijing ¹ ; Zhang, Weijun ⁴ ; Zhang, Xueyong ² ; Li, Tian ² ;

作者机构： 1.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Gene Resources & Germplasm Enhancemen, Beijing 100081, Peoples R China

3.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China

4.Ningxia Acad Agr & Forestry Sci, Crop Res Inst, Yinchuan 750002, Peoples R China

关键词： ABA response; drought tolerance; PP2A; TaTIP41; TaTAP46; TOR signaling; Triticum aestivum

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Drought is a major environmental stress limiting global wheat (Triticum aestivum) production. Exploring drought tolerance genes is important for improving drought adaptation in this crop. Here, we cloned and characterized TaTIP41, a novel drought tolerance gene in wheat. TaTIP41 is a putative conserved component of target of rapamycin (TOR) signaling, and the TaTIP41 homoeologs were expressed in response to drought stress and abscisic acid (ABA). The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect. Furthermore, TaTIP41 physically interacted with TaTAP46, another conserved component of TOR signaling. Like TaTIP41, TaTAP46 positively regulated drought tolerance. Furthermore, TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities. Silencing TaPP2A-2 improved drought tolerance in wheat. Together, our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat, and their potential application in improving wheat environmental adaptability.