文献类型： 外文期刊

作者： Zhi, Ai-min ¹ ; Zhou, Xiang-yan ³ ; Zuo, Jian-jun ¹ ; Zou, Shi-geng ¹ ; Huang, Zhi-yi ¹ ; Wang, Xiao-lan ¹ ; Tao, Lin; ¹ ;

作者机构： 1.S China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.ASIAPAC DongGuan Biotecnol Co Ltd, Dongguan 523808, Peoples R China

关键词： tissue distribution

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

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收录情况： SCI

摘要： In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y+LAT I and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine y(+)LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat y(+)LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest y(+)LAT1 mRNA abundance and the lung had the lowest y(+)LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine y(+)LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.