文献类型： 外文期刊

作者： Wang, Ke ¹ ; Zhang, Yuelang ² ; Han, Xiaotao ¹ ; Wu, Qun ¹ ; Liu, Hu ¹ ; Han, Jiancheng ¹ ; Zhou, Hanlin ¹ ; Davis, Michael E. ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Zhanjiang Expt Stn, Zhanjiang 524013, Peoples R China

2.Zhejiang Univ, Hainan Inst, Sanya 572024, Peoples R China

关键词： PLEC; copy number variation; goats; growth traits; meat quality

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2023 年 13 卷 23 期

页码：

收录情况： SCI

摘要： Simple Summary: As the global population continues to grow and the demand for unique meat flavor increases, goat meat has emerged as a sustainable choice for meat consumption. However, improving goat meat production and quality has become a pressing challenge for the industry. The PLEC gene has multiple regulatory effects on muscle development. Our previous study revealed that the PLEC gene in Leizhou black goats has numerous structural variations. In this study, three CNVs were identified in 417 Leizhou black goats. PLEC CNV-1 showed a significant association with chest circumference, body weight, carcass weight, the cross-sectional area of the longissimus dorsi lumbar muscle, and shear stress (p < 0.05). The gain type is significantly associated with PLEC expression in muscle (p <0.01). These findings suggest that PLEC CNV-1 plays a key role in the growth and muscle development of Leizhou black goats, highlighting its potential as a valuable molecular marker for genetic improvement in goat breeding programs. The plectin (PLEC) gene is crucial in regulating muscle development and maintaining the cytoskeleton. An abnormal expression of PLEC can lead to muscle atrophy and muscular dystrophy. In a previous study, we found that Leizhou black goats exhibit abundant structural variations in the PLEC gene. However, the genetic effects of these variations on growth traits and meat quality in goats are not fully understood. In this study, three PLEC copy number variations (CNVs) were identified in a population of 417 Leizhou black goats, using quantitative polymerase chain reaction (qPCR) technology. Population distribution analysis revealed a high abundance of various types of these three CNVs. PLEC mRNA was found to be highly expressed in muscle tissue and remained consistently high from 1 month to 24 months after birth. Specifically, the gain type of CNV-1 (chr14: 81056401-81064800) showed a significant association with PLEC mRNA expression in muscle tissue (p < 0.01). The sequence of CNV-1 in PLEC shares similarities with three domain superfamilies associated with muscle development and skin disease. Furthermore, there were significant differences in chest circumference, body weight, carcass weight, the cross-sectional area of the longissimus dorsi lumbar muscle, and shear stress between different types of CNV-1 (p < 0.05). Notably, goats with the CNV-1 gain type demonstrated better phenotypic values compared to those with loss and normal types. These findings suggest that PLEC CNV-1 could play a crucial role in the growth and muscle development of Leizhou black goats, making it a potential marker for assisted selection in goat breeding.