文献类型： 外文期刊

作者： Tian, Yuzhen ¹ ; Wang, Xizi ¹ ; Huang, Huoqing ³ ; Deng, Xin ⁴ ; Zhang, Baihong ⁶ ; Meng, Yixuan ¹ ; Wu, Libo ¹ ; Chen, Hang ¹ ; Zhong, Yun ³ ; Chen, Wenli ¹ ;

作者机构： 1.South China Normal Univ, MOE Key Lab Laser Life Sci, Guangzhou 510631, Peoples R China

2.South China Normal Univ, Inst Laser Life Sci, Coll Biophoton, Sch Optoelect Sci & Engn,Guangdong Prov Key Lab La, Guangzhou 510631, Peoples R China

3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangdong Prov Key Lab Sci & Technol Res Fruit Tre, Minist Agr & Rural Affairs,Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

4.City Univ Hong Kong, Dept Biomed Sci, Hong Kong, Peoples R China

5.City Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China

6.Guangdong Acad Sci, Inst Nanfan & Seed Ind, Guangzhou 510640, Peoples R China

关键词： DnaJ; citrus; huanglongbing (HLB); disease resistance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 22 期

页码：

收录情况： SCI

摘要： Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide. The etiological agent responsible for this disease is "Candidatus Liberibacter asiaticus" (CLas), a phloem-restricted bacterium transmitted by psyllid vectors. To date, effective practical strategies for curing Citrus HLB remain elusive. Additionally, no susceptibility genes associated with HLB have been identified in Citrus species, thereby complicating the application of gene-editing techniques such as CRISPR-Cas9 to enhance resistance to HLB. The co-chaperone DnaJ plays a crucial role in protein folding and the regulation of various physiological activities, and it is also associated with multiple pathological processes. DnaJ has been extensively studied in many species, including Arabidopsis, rice, and wheat. However, there is limited information available regarding the DnaJ gene family in citrus. In this study, we conducted a comprehensive genome-wide analysis of the DnaJ family genes in various Citrus species. The Citrus genome was identified to contain 86 DnaJ genes, which were unevenly distributed across nine chromosomes. Phylogenetic analysis indicated that these genes could be classified into six distinct groups. Furthermore, transcriptomic analysis revealed that nine DnaJ genes exhibited significantly higher induction in HLB-infected samples relative to non-HLB-infected Citrus. Cis-acting elements within the promoters of DnaJ genes were also examined, revealing the presence of hormone and defense/stress responsiveness elements (TC-rich) distributed on the ClDJC24 gene. The results were validated using quantitative real-time PCR (qRT-PCR). Additionally, the silencing of ClDJC24 suggested that this gene negatively regulates disease resistance in Citrus. Our study provided useful clues for further functional characterization and constructed a theoretical foundation for disease-resistant breeding in Citrus.