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Physiological changes and transcriptional modulation of HIF-alpha s in Siberian sturgeon in response to hypoxia

文献类型： 外文期刊

作者： Wang, Xiao-wen 1 ; Xiao, Shu-sheng 1 ; Zhang, Rong 1 ; Liu, Lili 1 ; Zhu, Hua 1 ;

作者机构： 1.Beijing Fisheries Res Inst, Beijing 100068, Peoples R China

2.Beijing Key Lab Fishery Biotechnol, Beijing 100068, Peoples R China

3.Natl Freshwater Fisheries Engn Technol Res Ctr, Beijing 100068, Peoples R China

关键词： Hypoxia; Acipenser baeri; HIF-alpha s; qRT-PCR; Hematology indices

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2021 年 545 卷

页码：

收录情况： SCI

摘要： To clarify the effects of acute hypoxia on physiological response and mRNA expression of Hypoxia-inducible factors (HIFs) in Siberian sturgeon, a chondrostean fish, severe hypoxia treatment was implemented by transfer of sturgeons from normoxia (DO = 7 mg/mL) to hypoxia (DO = 2 mg/mL). We found that the levels of serum cortisol, GLU, SOD, LDH, CK and CREA increased significantly at the early time-points post hypoxia exposure. Whereas the activity of immune related enzyme - AKP showed a sudden drop, followed by a recovery. Further, we identified and characterized three hif-alpha s cDNA sequences, namely Abhif-1 alpha, Abhif-2 alpha, and Abhif-3 alpha. The mRNA abundance of three hif-alpha s was all rich in brain, heart and liver. During acute hypoxia exposure, Abhif-1 alpha and Abhif-3 alpha mRNA expression levels increased significantly in the heart, gill and brain at early time-points, and gradually declined later on. Abhif-2 alpha only showed a slight increase in the gill upon hypoxia stress. In conclusion, we observed that, acute hypoxia affected the muscle function and immune related enzyme activity of Acipenser baeri; in response to hypoxia stress, sturgeons enhanced antioxidant capacity, anaerobic metabolism and up regulated the transcriptional expression of Abhif-1 alpha and Abhif-3 alpha. Our results might be helpful for further understanding of the potential effect of hypoxia on chondrostean fish and their molecular anti-hypoxia mechanisms.

  • 相关文献

[1]Physiological changes and transcriptional modulation of HIF-alpha s in Siberian sturgeon in response to hypoxia. Wang, Xiao-wen,Xiao, Shu-sheng,Zhang, Rong,Liu, Lili,Zhu, Hua,Wang, Xiao-wen,Xiao, Shu-sheng,Zhang, Rong,Liu, Lili,Zhu, Hua,Wang, Xiao-wen,Xiao, Shu-sheng,Zhang, Rong,Liu, Lili,Zhu, Hua. 2021

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