Expression of ZjPSY, a Phytoene Synthase Gene from Zoysia japonica Affects Plant Height and Photosynthetic Pigment Contents
文献类型： 外文期刊
作者： Dong, Di 1 ; Zhao, Yuhong 2 ; Teng, Ke 1 ; Tan, Penghui 4 ; Liu, Zhuocheng 1 ; Yang, Zhuoxiong 1 ; Han, Liebao 1 ; Chao, Yuehui 1 ;
作者机构： 1.Beijing Forestry Univ, Sch Grassland Sci, Beijing 100083, Peoples R China
2.Tibet Agr & Anim Husb Univ, Anim Sci Coll, Nyingchi 860000, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Beijing 100083, Peoples R China
4.Beijing Chaoyang Foreign Language Sch, Beijing 100101, Peoples R China
关键词： carotenoids; dwarfing; Zoysia japonica
期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）
ISSN：
年卷期： 2022 年 11 卷 3 期
页码：
收录情况： SCI
摘要： Phytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, ZjPSY was isolated and identified from Zoysia japonica, an important lawn grass species. ZjPSY cDNA was 1230 bp in length, corresponding to 409 amino acids. ZjPSY showed higher expression in young leaves and was downregulated after GA(3), ABA, SA, and MeJA treatments, exhibiting a sensitivity to plant hormones. Regulatory elements of light and plant hormone were found in the upstream of ZjPSY CDS. Expression of ZjPSY in Arabidopsis thaliana protein led to carotenoid accumulation and altered expression of genes involved in the carotenoid pathway. Under no-treatment condition, salt treatment, and drought treatment, transgenic plants exhibited yellowing, dwarfing phenotypes. The carotenoid content of transgenic plants was significantly higher than that of wild-type under salt stress and no-treatment condition. Yeast two-hybrid screening identified a novel interacting partner ZjJ2 (DNAJ homologue 2), which encodes heat-shock protein 40 (HSP40). Taken together, this study suggested that ZjPSY may affect plant height and play an important role in carotenoid synthesis. These results broadened the understanding of carotenoid synthesis pathways and laid a foundation for the exploration and utilization of the PSY gene.
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