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PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge

文献类型： 外文期刊

作者： Zhao, Yongxiang 1 ; Fan, Baochao 1 ; Song, Xu 1 ; Gao, Jie 1 ; Guo, Rongli 1 ; Yi, Cheng 6 ; He, Zhaoming 6 ; Hu, Hongpeng 6 ; Jiang, Jianhao 6 ; Zhao, Lixiang 6 ; Zhong, Tianyi 6 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Peoples R China

2.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing, Peoples R China

3.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing, Peoples R China

4.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

5.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou, Peoples R China

6.Suzhou Huiliao Biomed Technol Co Ltd, Suzhou, Peoples R China

7.Soochow Univ, Suzhou Med Coll, Suzhou, Peoples R China

关键词： mRNA vaccine; PEDV; spike protein; protective immunity; piglets

期刊名称：MBIO （ 影响因子：6.4； 五年影响因子：6.9 ）

ISSN： 2150-7511

年卷期： 2024 年 15 卷 2 期

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV), a swine enteropathogenic coronavirus, causes severe diarrhea in neonatal piglets, which is associated with a high mortality rate. Thus, developing effective and safe vaccines remains a top priority for controlling PEDV infection. Here, we designed two lipid nanoparticle (LNP)-encapsulated mRNA (mRNA-LNP) vaccines encoding either the full-length PEDV spike (S) protein or a multiepitope chimeric spike (Sm) protein. We found that the S mRNA-LNP vaccine was superior to the Sm mRNA-LNP vaccine at inducing antibody and cellular immune responses in mice. Evaluation of the immunogenicity and efficacy of the S mRNA vaccine in piglets confirmed that it induced robust PEDV-specific humoral and cellular immune responses in vivo. Importantly, the S mRNA-LNP vaccine not only protected actively immunized piglets against PEDV but also equipped neonatal piglets with effective passive anti-PEDV immunity in the form of colostrum-derived antibodies after the immunization of sows. Our findings suggest that the PEDV-S mRNA-LNP vaccine is a promising candidate for combating PEDV infection.IMPORTANCEPorcine epidemic diarrhea virus (PEDV) continues to harm the global swine industry. It is important to develop a highly effective vaccine to control PEDV infection. Here, we report a PEDV spike (S) mRNA vaccine that primes a potent antibody response and antigen-specific T-cell responses in immunized piglets. Active and passive immunization can protect piglets against PED following the virus challenge. This study highlights the efficiency of the PEDV-S mRNA vaccine and represents a viable approach for developing an efficient PEDV vaccine. Porcine epidemic diarrhea virus (PEDV) continues to harm the global swine industry. It is important to develop a highly effective vaccine to control PEDV infection. Here, we report a PEDV spike (S) mRNA vaccine that primes a potent antibody response and antigen-specific T-cell responses in immunized piglets. Active and passive immunization can protect piglets against PED following the virus challenge. This study highlights the efficiency of the PEDV-S mRNA vaccine and represents a viable approach for developing an efficient PEDV vaccine.

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