您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of germ cells in large yellow croaker (Larimichthys crocea) and yellow drum (Nibea albiflora) using RT-PCR and in situ hybridization analyses

文献类型： 外文期刊

作者： Yu, Yanjie 1 ; Yang, Yang 2 ; Ye, Huan 4 ; Lu, Lei 1 ; Li, Haidong 1 ; Xu, Zhijin 5 ; Li, Weiye 5 ; Yin, Xiaolong 5 ; Xu, Dongdong 2 ;

作者机构： 1.Zhejiang Ocean Univ, Sch Fisheries, Zhoushan, Peoples R China

2.Zhejiang Marine Fisheries Res Inst, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan, Peoples R China

3.Zhejiang Ocean Univ, Ocean & Fisheries Res Inst, Zhoushan, Peoples R China

4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan, Peoples R China

5.Zhoushan Fisheries Res Inst, Zhoushan, Peoples R China

关键词： Germ cell transplantation; Vasa; Nanos2; Dnd; Species-specific primers; Species-specific probes

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 863 卷

页码：

收录情况： SCI

摘要： Ocean-caught large yellow croaker (Larimichthys crocea) represents an important germplasm resource for the breeding of this species; however, these fish tend to show poor survival in captivity and would be unsuitable breeding purposes. As an alternative to the use of wild-caught croakers, germ cell transplantation has been proposed using the L. crocea specimens as donors and yellow drum (Nibea albiflora) as recipients. In this regard, the identification of L. crocea and N. albiflora germ cells is an essential prerequisite for establishing a germ cell transplantation protocol for these fish. In this study, we cloned the 3MODIFIER LETTER PRIME untranslated regions (UTR) of the vasa, dnd, and nanos2 genes in N. albiflora using the rapid amplification of cDNA ends (RACE) method and then aligned and analyzed the sequences of the corresponding genes in L. crocea and N. albiflora. On the basis of gene sequence differences, we designed species-specific primers and probes for RT-PCR analysis and in situ hybridization. RT-PCR analysis revealed that these species-specific primers exclusively amplified DNA from gonads of the respective species, thus confirming that we had six specific primer pairs that could be used to distinguish the germ cells in L. crocea and N. albiflora. Using in situ hybridization analysis, we established that whereas Lcvasa and Nadnd probes showed high species specificity, the probes for Navasa and Lcdnd showed a less specificity. In situ hybridization using Lcvasa and Nadnd thus enabled us to visualize the germ cells in these two species. Using these species-specific primers and probes, we can reliably distinguish the germ cells of L. crocea and N. albiflora, thereby establishing an effective approach for the post-transplantation identification of germ cells when using L. crocea and N. albiflora as donors and recipients, respectively.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台