Identification of miRNAs Involved in Olfactory Regulation in Antennae of Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae)
文献类型： 外文期刊
作者： Zhang, Yu 1 ; Li, Yanyan 2 ; Han, Haibin 2 ; Wang, Xiaoling 3 ; Gao, Shujing 1 ; Zhao, Qing 1 ; Bieerdebieke, Halima 4 ; Xu, Linbo 1 ; Zang, Qicong 5 ; Wang, Hui 1 ; Bai, Penghua 6 ; Lin, Kejian 1 ;
作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Biohazard Monitoring Green Prevent & Contr, Inst Grassland Res, Hohhot 010010, Peoples R China
2.Inner Mongolia Agr Univ, Res Ctr Grassland Entomol, Hohhot 010020, Peoples R China
3.Xilin Gol League Agr & Anim Husb Technol Promot Ct, Xilinhot 026000, Peoples R China
4.Xinjiang Uygur Autonomous Reg Prod Qual Supervis &, Urumqi 830049, Peoples R China
5.Heilongjiang Prov Grassland Stn, Harbin 150069, Peoples R China
6.Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin 300384, Peoples R China
关键词：
期刊名称：LIFE-BASEL （ 影响因子：3.4； 五年影响因子：3.4 ）
ISSN：
年卷期： 2024 年 14 卷 12 期
页码：
收录情况： SCI
摘要： The beet webworm, Loxostege sticticalis, is a typical migratory pest. Although miRNAs participate in many physiological functions, little is known about the functions of miRNAs in olfactory regulation. In this study, 1120 (869 known and 251 novel) miRNAs were identified in the antennae of L. sticticalis by using high-throughput sequencing technology. Among the known miRNAs, 189 from 49 families were insect-specific, indicating that these miRNAs might play unique roles in insects. Furthermore, based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we found that 3647 and 1393 miRNAs were associated with localization and the regulation of localization, respectively, and 80 miRNAs were enriched in the neuroactive ligand-receptor interaction pathway. These miRNAs might be involved in the olfactory system of L. sticticalis. Notably, qRT-PCR showed that most of the tested miRNAs presented similar expression patterns compared with the RNA-seq data and that miR-87-3, novel-miR-78, and novel-miR-142 were significantly differentially expressed in the antennae of males and females. In addition, 21 miRNAs were predicted to target 23 olfactory genes, including 10 odorant-binding proteins (OBPs), 3 chemosensory proteins (CSPs), 4 odorant receptors (ORs), 1 ionotropic receptor (IR), and 5 gustatory receptors (GRs). The olfactory-related miRNAs exhibited low-abundance transcripts, except undef-miR-55 and undef-miR-523, and gender-biased expression was not observed for olfactory-related miRNAs. Our findings provide an overview of the potential miRNAs involved in olfactory regulation, which may provide important information on the function of miRNAs in the insect olfactory system.
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