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Identification and detection of the V1848I indoxacarb resistance mutation in the beet armyworm, Spodoptera exigua

文献类型： 外文期刊

作者： Zhang, Xianxia 1 ; Zhang, Ruiming 2 ; Yu, Mengqi 2 ; Liu, Rui 2 ; Liu, Naijing 2 ; Teng, Haiyuan 4 ; Pei, Yakun 2 ; Hu, Zhaonong 2 ; Zuo, Yayun 1 ;

作者机构： 1.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

2.Northwest A&F Univ, Coll Plant Protect, Key Lab Plant Protect Resources & Pest Management, Minist Educ,Key Lab Integrated Pest Management Loe, Yangling 712100, Shaanxi, Peoples R China

3.Key Lab Bot Pesticide R&D Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China

4.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai 201403, Peoples R China

关键词： Beet armyworm; Indoxacarb; Resistance; Mutations; AS-PCR; Insecticide resistance management

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN： 0048-3575

年卷期： 2024 年 203 卷

页码：

收录情况： SCI

摘要： Indoxacarb is a pivotal insecticide used worldwide to manage Spodoptera exigua , a devastating agricultural pest. This active compound plays a crucial role in resistance management strategies due to its distinctive mode of action. A field population of S. exigua (SH23) from Shanghai, China, exhibited significantly reduced susceptibility to indoxacarb, with a resistance ratio of 113.84 -fold in biological assays. Following two rounds of laboratory screening with indoxacarb, the resistance of the new strain (SH23-S2) escalated steeply to 876.15 -fold. Genetic analyses of both the SH23 and SH23-S2 strains demonstrated autosomal inheritance and incompletely dominant resistance patterns. Synergist assays indicated a minor role of detoxification enzymes (glutathione s-transferases and cytochrome P450) of SH23-S2 strain in this resistance, implicating target -site resistance as the primary mechanism. To explore the impact of target -site resistance, segment 1 -6 of domain IV (IVS1 -6) of the sodium channel in S. exigua was cloned, and the sequences from susceptible and indoxacarb-resistant S. exigua were compared. The V1848I mutation, linked to indoxacarb resistance in Plutella xylostella , Tuta absoluta and Liriomyza trifolii , was identified and strongly associated with the indoxacarb-resistant phenotype in the S. exigua SH23-S2 strain, whereas the F1845Y mutation was not detected. Furthermore, a molecular test for the V1848I mutation in field populations was created using an allele -specific PCR (AS-PCR). The discovery of indoxacarb resistance mutation and the creation of diagnostic tool will enable the early detection of indoxacarb resistance, which will facilitate the implementation of targeted resistance management strategies, ultimately delaying the proliferation of resistance.

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