Integrated Transcriptome and Metabolome Analysis Elucidates the Defense Mechanisms of Pumpkin Against Gummy Stem Blight
文献类型： 外文期刊
作者： Zhao, Qian 1 ; Zhang, Liyan 2 ; Han, Weibo 3 ; Wang, Ziyu 3 ; Wu, Jianzhong 3 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Cultivat & Farming Res Inst, Harbin 150086, Peoples R China
2.Inner Mongolia Agr Univ, Forestry Coll, Hohhot 010011, Peoples R China
3.Heilongjiang Acad Agr Sci, Inst Forage & Grassland Sci, Harbin 150086, Peoples R China
关键词：
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 6 期
页码：
收录情况： SCI
摘要： Gummy stem blight (GSB) is a pervasive disease that causes considerable economic losses in cucurbit crops and poses a significant threat to pumpkin production. However, the molecular interaction mechanisms between pumpkin and the pathogen remain largely unexplored. In our previous research, we isolated and identified Stagonosporopsis cucurbitacearum (Sc) as the primary causative agent of pumpkin stem blight in Northeast China. Through whole-genome analysis, we identified several pathogenic genes associated with Sc infection in pumpkins. In this study, we performed a comprehensive comparative transcriptomic and metabolomic analysis of unvaccinated and Sc-inoculated pumpkins. We observed distinct differences in gene expression profiles, with these genes being significantly enriched in pathways related to plant-pathogen interactions, phytohormone signal transduction, and metabolic processes, including phenylpropanoid biosynthesis. Joint analysis revealed that the phenylpropanoid biosynthesis pathway was activated in Sc-infected pumpkins. Notably, two metabolites involved in the phenylpropanoid and flavonoid biosynthesis pathways, p-coumaric acid and quercetin, exhibited significant upregulation, suggesting their potential roles in conferring resistance to GSB. These findings enhance our understanding of the molecular mechanisms underlying the defense response against GSB infection in pumpkins and may provide valuable insights for developing strategies to control GSB disease.
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