文献类型： 外文期刊
作者： Li, Jikai 1 ; Chai, Lu 2 ; Yang, Mei 2 ; Zhang, Hailing 1 ; Shang, Chen 1 ; Liu, Yuxuan 2 ; Qian, Kailin 2 ; Sun, Jiuding 2 ; Han, Weibo 1 ; Zhang, Pan 2 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Inst Grass Res, Harbin, Peoples R China
2.Northeast Agr Univ, Coll Anim Sci & Technol, Dept Grassland Sci, Harbin, Peoples R China
关键词：
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2025 年 16 卷
页码：
收录情况： SCI
摘要： Low-temperature stress is a major environmental factor that limits the yield, quality, and geographical distribution of forage crops and restricts the development of the forage industry. As a core component of plant circadian clocks, Late Elongated Hypocotyl (LHY) plays a crucial role in regulating plant rhythms and responses to abiotic stress. However, the molecular mechanism by which LHY regulates the cold tolerance of alfalfa has not been reported. In this study, MsLHY, which is 2,235 bp in length and encodes 744 amino acids, was isolated from alfalfa. MsLHY was highly expressed in roots and stems and was significantly induced by low temperature. Transgenic MsLHY-overexpressing (OE) and RNAi alfalfa plants were obtained via Agrobacterium-mediated transformation. Under low-temperature stress, OE plants presented reduced reactive oxygen species accumulation and more osmotic regulatory substances, as well as greater antioxidant enzyme activity, to combat cold stress. Conversely, the RNAi plants presented trends opposite those of the OE plants. Furthermore, under cold stress, the overexpression of MsLHY upregulated the expression of the cold-responsive genes MsICE1, MsCBF1, MsCOR15A, and MsCML10, as well as the expression of the antioxidant-synthesizing genes MsSOD1 and MsCAT1, thereby increasing the cold tolerance of transgenic alfalfa. These results suggest that MsLHY plays an important role in increasing the cold tolerance of alfalfa.
- 相关文献
作者其他论文 更多>>
-
The synergistic roles of MsRCI2B and MsRCI2E in the regulation of ion balance and ROS homeostasis in alfalfa under salt stress
作者：Zhang, Depeng;He, Pin;Li, Donghuan;Meng, Jing;Luo, Yaqin;Wang, Xinsheng;Zhang, Shichao;Cai, Hua;Shen, Zhongbao;Wang, Jianli;Zhang, Dongmei;You, Jia;Zhuang, Xu;Mu, Linlin;Han, Weibo
关键词：Medicago sativa; RCI2; Salt stress
-
Integrative analysis of transcriptome and metabolism reveals functional roles of redox homeostasis in low light and salt combined stress in Leymus chinensis
作者：Li, Jikai;Zhang, Hailing;Shang, Chen;Han, Weibo;Li, Jikai;Fang, Suyang;Iqbal, Zubair;Huang, Kai;Meng, Xiangshen;Dai, Muyuan;Lu, Zhiheng;Guo, Bingnan;Qu, Mingnan
关键词：Sheepgrass; Transcriptome; Photorespiration; ROS; Carbon metabolism; Antioxidant; TCA
-
Integrated Transcriptome and Metabolome Analysis Elucidates the Defense Mechanisms of Pumpkin Against Gummy Stem Blight
作者：Zhao, Qian;Zhang, Liyan;Han, Weibo;Wang, Ziyu;Wu, Jianzhong
关键词：
Stagonosporopsis cucurbitacearum ; DAMs; DEGs; transcriptome and metabolome analysis; plant-pathogen interaction -
Transcriptomic Analysis Identifies Molecular Response of the Tolerant Alfalfa (Medicago sativa) Cultivar Nongjing 1 to Saline-Alkali Stress
作者：Zhang, Dongmei;Shen, Zhongbao;Han, Weibo;Mou, Linlin;Zhuang, Xu;Wang, Jianli;Li, Jinxia;Zhang, Yiming;Zhang, Yuanhao;Wang, Wenhui;Li, Zhaohui;Zhu, Peng;Huang, Yongshun;Han, Long;Wang, Mingyu;Zhang, Zijian;Pang, Qiuying;Li, Lixin;Li, Jinxia
关键词：
Medicago sativa ; saline-alkali adaptation; yield; flavonoid biosynthesis; transcriptional regulation -
QTL Mapping and Candidate Gene Screening for Enhancing Oil Content in Silage Maize
作者：Wu, Jianzhong;Han, Weibo;Sun, Dequan;Shen, Zhongbao;Wang, Qi;Zhao, Qian
关键词：silage maize; oil content; QTL; transcriptome; gene mining
-
The synergistic roles of MsRCI2B and MsRCI2E in the regulation of ion balance and ROS homeostasis in alfalfa under salt stress (vol 300, 140093, 2025)
作者：Zhang, Depeng;He, Pin;Li, Donghuan;Meng, Jing;Luo, Yaqin;Wang, Xinsheng;Zhang, Shichao;Cai, Hua;Shen, Zhongbao;Wang, Jianli;Zhang, Dongmei;You, Jia;Zhuang, Xu;Mu, Linlin;Han, Weibo
关键词：
-
Adaptability evaluation of four alfalfa (Medicago sativa L.) varieties in cold regions of northern China: Based on multidimensional indicators, metabolites, and soil microbial community analysis
作者：He, Ying;Zhao, Yuzi;Niu, Jiayi;Tian, Lijuan;Li, Yonggang;Li, Jiekai;Wang, Ziyu;Wu, Jianzhong;Han, Weibo
关键词：Medicago sativa; Cold adaptation; Multidimensional evaluation framework; Rhizosphere microbiome-metabolome crosstalk; Fuzzy membership analysis
