An efficient root transformation system for CRISPR/Cas9-based analyses of shoot-root communication in cucurbit crops
文献类型： 外文期刊
作者： Geng, Shouyu 1 ; Sohail, Hamza 1 ; Cao, Haishun 1 ; Sun, Jingyu 1 ; Chen, Zhi 1 ; Zhou, Lijian 1 ; Wang, Wenbo 1 ; Ye, Runwen 1 ; Yang, Li 1 ; Bie, Zhilong 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China
2.Guangdong Acad Agr Sci, Inst Facil Agr, Guangzhou 510640, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.291； 五年影响因子：7.487 ）
ISSN： 2662-6810
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： Cucurbit crops are suitable models for studying long-distance signaling in horticultural plants. Although thousands of substances are graft transmissible in cucurbits, functional studies have been hampered by the lack of efficient genetic transformation systems. Here, we report a convenient and efficient root transformation method for several cucurbit crops that will facilitate studies of functional genes and shoot-root crosstalk. We obtained healthy plants with completely transformed roots and non-transgenic shoots within 6 weeks. Furthermore, we combined this root transformation method with grafting, which allowed for gene manipulation in the rootstock. We validated our system by exploring salt tolerance mechanisms using a cucumber (Cucumis sativus)/pumpkin (Cucurbita moschata Duch.) (scion/rootstock) graft in which the sodium transporter gene High-affinity K+ transporter1 (CmoHKT1;1) was edited in the pumpkin rootstock and by overexpressing the pumpkin tonoplast Na+/H+ antiporter gene Sodium hydrogen exchanger4 (CmoNHX4) in cucumber roots.
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