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Transcriptomic and functional characterization reveals CsHAK5;3 as a key player in K plus homeostasis in grafted cucumbers under saline conditions

文献类型： 外文期刊

作者： Peng, Yuquan 1 ; Cao, Haishun 1 ; Peng, Zhaowen 1 ; Zhou, Lijian 1 ; Sohail, Hamza 1 ; Cui, Lvjun 1 ; Yang, Li 1 ; Huang, Yuan 1 ; Bie, Zhilong 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Minist Educ, Wuhan, Peoples R China

2.Inst Facil Agr, Guangdong Acad Agr Sci, Guangzhou, Peoples R China

关键词： Grafted cucumber; Salinity; K plus homeostasis; HAK5; Transcriptome

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2023 年 326 卷

页码：

收录情况： SCI

摘要： Grafting can improve the salt tolerance of many crops. However, critical genes in scions responsive to rootstock under salt stress remain a mystery. We found that pumpkin rootstock decreased the content of Na+ by 70.24 %, increased the content of K+ by 25.9 %, and increased the K+/Na+ ratio by 366.0 % in cucumber scion leaves. RNA-seq analysis showed that ion transport-related genes were the key genes involved in salt stress tolerance in grafted cucumber. The identification and analysis of the expression of K+ transporter proteins in cucumber and pumpkin revealed six and five HAK5 members, respectively. The expression of CsHAK5;3 in cucumber was elevated in different graft combinations under salt stress and most notably in cucumber scion/pumpkin root-stock. CsHAK5;3 was localized to the plasma membrane, and a yeast complementation assay revealed that it can transport K+. CsHAK5;3 knockout in hairy root mutants decreased the K+ content of leaves (45.6 %) and roots (50.3 %), increased the Na+ content of leaves (29.3 %) and roots (34.8 %), and decreased the K+/Na+ ratio of the leaves (57.9 %) and roots (62.9 %) in cucumber. However, CsHAK5;3 overexpression in hairy roots increased the K+ content of the leaves (31.2 %) and roots (38.3 %), decreased the Na+ content of leaves (17.2 %) and roots (14.3 %), and increased the K+/Na+ ratio of leaves (58.9 %) and roots (61.6 %) in cucumber. In conclusion, CsHAK5;3 in cucumber can mediate K+ transport and is one of the key target pumpkin genes that enhance salt tolerance of cucumber grafted.

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