A Virulent Trueperella pyogenes Isolate, Which Causes Severe Bronchoconstriction in Porcine Precision-Cut Lung Slices
文献类型： 外文期刊
作者： Qin, Lei 1 ; Meng, Fandan 1 ; He, Haijuan 3 ; Yang, Yong-Bo 1 ; Wang, Gang 1 ; Tang, Yan-Dong 1 ; Sun, Mingxia 1 ; Zhang, Wenlong 2 ; Cai, Xuehui 1 ; Wang, Shujie 1 ;
作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin, Peoples R China
2.Northeast Agr Univ, Coll Vet Med, Harbin, Peoples R China
3.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin, Peoples R China
关键词： Trueperella pyogenes; porcine precision-cut lung slices; virulent; bronchoconstriction; infection
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）
ISSN：
年卷期： 2022 年 8 卷
页码：
收录情况： SCI
摘要： Trueperella pyogenes causes disease in cattle, sheep, goats and swine, and is involved occasionally in human disease worldwide. Most reports implicating T. pyogenes have been associated with clinical cases, whereas no report has focused on pathogenicity of T. pyogenes in mouse models or precision-cut lung slice (PCLS) cultures from swine. Here, we isolated and identified a virulent, beta-hemolytic, multidrug-resistant T. pyogenes strain named 20121, which harbors the virulence marker genes fimA, fimE, nanH, nanP and plo. It was found to be highly resistant to erythromycin, azithromycin and medemycin. Strain 20121 was pathogenic in mouse infection models, displaying pulmonary congestion and inflammatory cell infiltration, partial degeneration in epithelial cells of the tracheal and bronchiolar mucosa, a small amount of inflammatory cell infiltration in the submucosa, and bacteria (>10(4) CFU/g) in the lung. Importantly, we used T. pyogenes 20121 to infect porcine precision-cut lung slices (PCLS) cultures for the first time, where it caused severe bronchoconstriction. Furthermore, dexamethasone showed its ability to relieve bronchoconstriction in PCLS caused by T. pyogenes 20121, highlighting dexamethasone may assist antibiotic treatment for clinical T. pyogenes infection. This is the first report of T. pyogenes used to infect and cause bronchoconstriction in porcine PCLS. Our results suggest that porcine PCLS cultures as a valuable 3D organ model for the study of T. pyogenes infection and treatment in vitro.
- 相关文献
作者其他论文 更多>>
-
Prevalence, Time of Infection, and Diversity of Porcine Reproductive and Respiratory Syndrome Virus in China
作者：Li, Chaosi;Fan, Aihua;Huang, Lv;Liu, Zhicheng;Zhang, Jianfeng;Liu, Zhicheng;Wang, Gang;Zhou, Lei;Zhou, Lei;Zhang, Hongliang;Zhang, Zhendong;Zhang, Yan
关键词：PRRSV; prevalence; three-sample strategy; time of infection; wild strain diversity
-
Multivalent nanobody-based sandwich enzyme-linked immunosorbent assay for sensitive detection of porcine reproductive and respiratory syndrome virus
作者：Sun, Mingxia;Sun, Yue;Yang, Yongbo;Zhao, Man;Zhang, Minmin;Xia, Dasong;Wang, Tao;Gao, Yanfei;Wang, Shanghui;Wang, Haiwei;Cai, Xuehui;An, Tongqing;An, Tongqing;Cai, Xuehui;Cao, Dan;Cai, Xuehui;An, Tongqing
关键词：PRRSV; Multivalent nanobody; Nanobody-HRP; Sandwich ELISA
-
Immunogenicity Characterization of the Recombinant gI Protein Fragment from Pseudorabies Virus and an Evaluation of Its Diagnostic Use in Pigs
作者：He, Haijuan;Qi, Baojie;Yang, Yongbo;Chen, Tianfeng;Cai, Xuehui;An, Tongqing;Wang, Shujie;He, Haijuan;Cui, Xiaowen;Cai, Xuehui;Wang, Shujie
关键词：pseudorabies virus; envelope glycoprotein I; immunogenicity; Ni-NTA chromatography; ELISA
-
Inflammation plays a critical role in damage to the bronchiolar epithelium induced by Trueperella pyogenes in vitro and in vivo
作者：Qin, Lei;Meng, Fandan;Li, Siqi;Zhang, Hongliang;Sun, Yuan;An, Tongqing;Cai, Xuehui;Wang, Shujie;Qin, Lei;He, Haijuan;Zhang, Wenlong;Cai, Xuehui;Wang, Shujie
关键词：Trueperella pyogenes; PBECs; PCLS; infection; inflammation; intracellular pathways
-
Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway
作者：He, Xinmiao;Tian, Ming;Wang, Wentao;Feng, Yanzhong;Chen, Heshu;He, Haijuan;Zhang, Haifeng;Liu, Di;Qin, Ziliang;Teng, Ran;Liu, Di;Jiang, Xinpeng
关键词：Min pig; follicle stem cell; BMP; Wnt
-
A Novel Motif in the 3 '-UTR of PRRSV-2 Is Critical for Viral Multiplication and Contributes to Enhanced Replication Ability of Highly Pathogenic or L1 PRRSV
作者：Xiong, Junyao;Cui, Xingyang;Zhao, Kuan;Wang, Qian;Huang, Xinyi;Li, Dongyan;Yu, Fang;Yang, Yongbo;Tian, Zhijun;Cai, Xuehui;An, Tongqing;Liu, Di
关键词：HP-PRRSV; 3 '-UTR; secondary structure; motif; replication; epidemic
-
Does gut microbiota regulate brooding in geese?
作者：Liu, Guojun;Guo, Zhenhua;Liu, Di;Zhao, Xiuhua;Li, Manyu;Sun, Jinyan;He, Haijuan;Yu, Xiaolong;Meng, He;Zheng, Yuming;Gu, Lihong;Chen, Zhifeng;Ma, Zhigang;Chen, Xingyong;Hu, Fanghong
关键词：Brooding; goose; gut microbiota; microbiome; transcription group
