您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Downregulation of miR-192 Alleviates Oxidative Stress-Induced Porcine Granulosa Cell Injury by Directly Targeting Acvr2a

文献类型： 外文期刊

作者： Zhang, Jiaqing 1 ; Ren, Qiaoling 1 ; Chen, Junfeng 1 ; Lv, Lingyan 2 ; Wang, Jing 1 ; Shen, Ming 3 ; Xing, Baosong 1 ; Wang, Xianwei 4 ;

作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Peoples R China

2.Anim Husb Res Inst Guangxi, Nanning 530001, Peoples R China

3.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China

4.Henan Prov Anim Husb Gen Stn, Zhengzhou 450008, Peoples R China

关键词： oxidative stress; granulosa cell apoptosis; miRNA; GSPB2

期刊名称：CELLS （ 影响因子：7.666； 五年影响因子：7.677 ）

ISSN：

年卷期： 2022 年 11 卷 15 期

页码：

收录情况： SCI

摘要： Follicular atresia is primarily caused by breakdown to granulosa cells (GCs) due to oxidative stress (OS). MicroRNAs (miRNAs) elicit a defense response against environmental stresses, such as OS, by acting as gene-expression regulators. However, the association between miRNA expression and OS in porcine GCs (PGCs) is unclear. Here, we examined the impact of H2O2-mediated OS in PGCs through miRNA-Seq. We identified 22 (14 upregulated and 8 downregulated) and 33 (19 upregulated and 14 downregulated) differentially expressed miRNAs (DEmiRNAs) at 100 mu M and 300 mu M H2O2, respectively, compared with the control group. Among the DEmiRNAs, mi-192 was most induced by H2O2-mediated OS, and the downregulation of miR-192 alleviated PGC oxidative injury. The dual-luciferase reporter assay results revealed that miR-192 directly targeted Acvr2a. The Acvr2a level was found to be remarkably decreased after OS. Furthermore, grape seed procyanidin B2 (GSPB2) treatment significantly reduced the H2O2-induced upregulation of miR-192, and decreased PGC apoptosis and oxidative damage. Meanwhile, GSPB2 prevented an H2O2-induced increase in caspase-3 activity, which was enhanced by the application of the miR-192 inhibitor. These results indicate that GSPB2 protects against PGC oxidative injury via the downregulation of miR-192, the upregulation of Acvr2a expression, and the suppression of the caspase-3 apoptotic signaling pathway.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台