Preparation and Characterization of Polysaccharides From Grifola frondosa and Their Human Intestinal Flora-modulating Effect
文献类型： 外文期刊
作者： Tang, Meng-Ting 1 ; Liu, Weiming 2 ; Wang, Xingli 2 ; Wan, Cheng 1 ; Zhou, Tao 1 ; Liu, Wei 3 ;
作者机构： 1.Zhejiang Gongshang Univ, Sch Food Sci & Biotechnol, Key Lab Food Microbial Technol Zhejiang Prov, Hangzhou, Peoples R China
2.Zhejiang Biosan Biotech Co Ltd, Lishui, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Zhejiang Prov Key Lab Agr Microbi, Hangzhou, Peoples R China
关键词： Grifola frondosa polysaccharide; extraction method; intestinal flora; modulating effect
期刊名称：CHEMISTRY & BIODIVERSITY （ 影响因子：2.5； 五年影响因子：2.6 ）
ISSN： 1612-1872
年卷期： 2025 年
页码：
收录情况： SCI
摘要： A novel and efficient hydrogen peroxide/ascorbic acid-assisted extraction method for the preparation of Grifola frondosa polysaccharide (GFP) was developed, and two GFP fractions (GFP-H and GFP-L) with different molecular weights (Mws) were obtained by separation with ultrafiltration. Both high Mw component (GFP-H, Mw 396.4 kDa) and low Mw component (GFP-L, Mw 12.5 kDa) are possibly mainly comprised of -> 4)-beta-Glcp-(1 ->, -> 6)-alpha-Glcp-(1 ->, -> 6)-alpha-Galp-(1 ->, alpha-T-Glcp, -> 3,6)-alpha-Manp-(1 ->, and beta-D-GlcpA-(1 -> units. The physicochemical properties of GFPs, such as surface morphology, rheological properties, and thermal stability, were also analyzed. Their modulatory effects on human gut microbiota were evaluated using a simulated human intestinal fermentation model in vitro. The results showed that GFPs could be degraded by intestinal microorganisms and promoted the production of short-chain fatty acids such as acetic acid and butyric acid. Compared with GFP-L, GFP-H exhibited a better ability to increase the abundance of beneficial bacteria (e.g., Bifidobacterium, Megasphaera, and Lactobacillus) and reduce the abundance of harmful bacteria (e.g., Escherichia/Shigella, and Lachnoclostridium). This work revealed that Mw of GFP had a significant impact on the human gut microbiota-regulating effect. This study provided valuable information about the relationship between the structure of GFPs and their regulatory effect on intestinal flora.
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