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Development of a colloidal gold immunochromatographic strip for the simultaneous detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus

文献类型： 外文期刊

作者： Zhou, Jinzhu 1 ; Wu, Wei 4 ; Wang, Dandan 1 ; Wang, Wei 1 ; Chang, Xinjian 1 ; Li, Yunchuan 1 ; Li, Jizong 1 ; Fan, Baochao 1 ; Zhou, Junming 1 ; Guo, Rongli 1 ; Zhu, Xuejiao 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol Minist Agr, Jiangsu Key Lab Food Qual & Safety State Key Lab C, Nanjing, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

3.Guotai Taizhou Ctr Technol Innovat Vet Biol, Taizhou, Peoples R China

4.Fujian Agr & Forestry Univ, Fuzhou, Peoples R China; Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Peoples R China

关键词： porcine epidemic diarrhea virus; transmissible gastroenteritis virus; colloidal gold immunochromatographic strip assay; monoclonal antibodies; RT-PCR

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.0； 五年影响因子：5.1 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： In recent years, porcine diarrhea-associated viruses have caused significant economic losses globally. These viruses present similar clinical symptoms, such as watery diarrhea, dehydration, and vomiting. Co-infections with porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are common. For the rapid and on-site preliminary diagnosis on the pig farms, this study aimed to develop a colloidal gold immunochromatography assay (GICA) strip for the detection of PEDV and TGEV simultaneously. The GICA kit showed that there was no cross-reactivity with the other five common porcine viruses. With visual observation, the lower limits were approximately 104 TCID50/mL and 104 TCID50/mL for PEDV and TGEV, respectively. The GICA strip could be stored at 4 degrees C or 25 degrees C for 12 months without affecting its efficacy. To validate the GICA strip, 121 clinical samples were tested. The positive rates of PEDV and TGEV were 42.9 and 9.9%, respectively, and the co-infection rate of the two viruses was 5.8% based on the duplex GICA strip. Thus, the established GICA strip is a rapid, specific, and stable tool for on-site preliminary diagnosis of PEDV- and TGEV-associated diarrhea.

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