您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of the exosomes in the allantoic fluid of the chicken embryo

文献类型： 外文期刊

作者： Li, Ying 1 ; Qu, Hao 1 ; Ji, Jian 1 ; Wang, Yan 1 ; Liu, Tianfei 1 ; He, Jingyi 1 ; Wang, Jie 1 ; Shu, Dingming 1 ; Luo, Che 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Guangdong Publ Lab Anim Breeding & Nutr, State Key Lab Livestock & Poultry Breeding, Guangdong Key Lab Anim Breeding & Nutr,Inst Anim, 1 Dafeng 1st St, Guangzhou 510640, Guangdong, Peoples R China

关键词： chicken; allantoic fluid; exosomes; miRNAs; cell migration

期刊名称：CANADIAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：1.015； 五年影响因子：1.094 ）

ISSN： 0008-3984

年卷期： 2021 年 101 卷 2 期

页码：

收录情况： SCI

摘要： The embryo stage is critical for chicken development. Numerous studies have been conducted to clarify the dynamic changes and functions of various proteins and the composition of amino acids during embryo development. However, the physiological characteristics of extraembryonic fluid (allantoic and amniotic), especially allantoic fluid (AF), remain largely unexplored; furthermore, how information is transmitted from embryonic fluid is unknown. In this study, AF-derived exosomes ranging from 60 to 160 nm in diameter from fertilized eggs at 13 d of incubation of fast-growth chickens (WG chicken), medium-growth chickens (Silky N4 chicken), and slow-growth chickens (Huiyang Beard chicken) were isolated and purified by different ultra-centrifugations and further verified by transmission electron microscopy and a flow nano-analyzer. Expression of the exosomal positive biomarkers of ALIX and HSP70 as well as lack of the epithelium marker GRP78 was observed by Western blotting. In addition, small RNA sequencing revealed that AF-derived exosomes at 13 d of incubation contained a large number of known miRNAs (32.62%-65.83%). The top 10 most abundant and co-expressed miRNAs were primarily related to development, growth, and immunity. In addition, AF-derived exosomes promoted DF-1 cell migration. These findings broadened our understanding of the characteristic of AF-derived exosomes.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台