您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

RsLBD3 regulates the secondary growth of taproot by integrating auxin and cytokinin signaling in radish (Raphanus sativus L.)

文献类型： 外文期刊

作者： Dong, Junhui 1 ; Wang, Yan 1 ; Xu, Liang 1 ; Li, Bingshuang 1 ; Zhang, Xiaoli 1 ; Chen, Yinglong 3 ; Ying, Jiali 1 ; Chen, Sen 1 ; Cui, Feng 5 ; Liu, Liwang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Natl Key Lab Crop Genet & Germplasm Enhancement &, Key Lab Hort Crop Biol & Genet Improvement East Ch, Nanjing 210095, Peoples R China

2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

3.Univ Western Australia, UWA Inst Agr, Perth, WA 6009, Australia

4.Univ Western Australia, Sch Agr & Environm, Perth, WA 6009, Australia

5.Yangzhou Univ, Coll Hort & Landscape Architecture, Yangzhou 225009, Peoples R China

关键词： cytokinin; LBD3; natural variation; radish; vascular cambium

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.3； 五年影响因子：10.8 ）

ISSN： 1672-9072

年卷期： 2025 年 67 卷 7 期

页码：

收录情况： SCI

摘要： Radish (Raphanus sativus L.) is a globally important root vegetable crop known for its diverse varieties and unique taproot characteristics. The LBD (LATERAL ORGAN BOUNDARIES DOMAIN) gene family, specific to plants, plays a pivotal role in the development of lateral plant organs. Nonetheless, the precise biological functions and molecular regulatory mechanisms of LBD genes in radish taproot development remain largely unexplored. In this study, the RsLBD3 gene was identified as a potential candidate affecting taproot size in radish through a genome-wide association study. Further investigation revealed two insertions in the C-terminal region of RsLBD3, with insertion(363) notably enhancing the transcriptional activation capability of RsLBD3. It was observed that radish taproots with RsLBD3(Ins-363) haplotype displayed significantly greater length and weight compared to those with RsLBD3(Del-363) haplotype. RNA in situ hybridization and reverse transcription quantitative polymerase chain reaction analysis revealed that the RsLBD3 gene exhibits high expression level in the vascular cambium and is induced by cytokinin treatment. Silencing the RsLBD3 gene resulted in the inhibition of vascular cambium activity in the taproot, thereby impeding thickening. Exogenous cytokinin treatment could partially rescue the small-taproot phenotypes caused by RsLBD3 silencing. Moreover, RsARF5 (AUXIN RESPONSE FACTOR 5), RsRR7b (RESPONSE REGULATOR 7), and RsCYCD3-1 (CYCLIN D3;1) were identified as target genes of RsLBD3. Notably, RsARF5 was found to directly regulate the expression of RsWOX4 (WUSCHEL-RELATED HOMEOBOX 4). Additionally, biochemical analysis demonstrated that RsTCP14 interacts with RsLBD3, contributing to the binding of RsLBD3 to its target genes. Collectively, these findings contribute to a better understanding of the regulatory mechanisms underlying taproot morphogenesis, and provide novel allelic variations for the genetic enhancement of taproot shape traits in radish.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台