Epmedin C Alleviates Deoxynivalenol-Induced Immunotoxicity by Inhibiting Caspase-1 Activation in Chicken Macrophages
文献类型： 外文期刊
作者： Bu, Wandi 1 ; Wu, Xinyao 1 ; Zhong, Zhihang 1 ; Li, Yuekai 1 ; Fang, Yaping 1 ; Chen, Shuang 1 ; Wang, Zige 1 ; Hong, Ying 1 ; Dai, Feiyang 3 ; Wang, Yan 4 ; Jiang, Jun 1 ; Deng, Yiqun 1 ;
作者机构： 1.South China Agr Univ, State Key Lab Swine & Poultry Breeding Ind, Guangzhou 510642, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Guangzhou 510640, Guangdong, Peoples R China
3.South China Agr Univ, Coll Life Sci, Guangdong Prov Key Lab Dev Biol & Environm Adaptat, Guangzhou 510642, Guangdong, Peoples R China
4.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
关键词： deoxynivalenol; chickens; caspase-1; macrophage; epmedin C
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Deoxynivalenol (DON), a prevalent mycotoxin in poultry feed, exerts immunotoxicity even at low doses, yet its mechanisms in poultry remain unclear. Here, we evaluated DON's effects using chicken macrophage HD11 cells and one-day-old chicks. In vitro, DON activated the caspase-1/IL-1 beta pathway, increased reactive oxygen species (ROS), and promoted proinflammatory cytokine release, impairing antibody production. Network pharmacology and molecular docking identified epmedin C, a major flavonoid from Epimedium, as a potential caspase-1 inhibitor. Epmedin C bound strongly to caspase-1, inhibited its activation, reduced ROS, and suppressed cytokine secretion in HD11 cells, while coculture assays confirmed restoration of antibody production. In vivo, dietary epmedin C supplementation alleviated DON-induced immunotoxicity by restoring antibody levels, reducing splenic caspase-1 activity, and preserving immune and intestinal structures. These findings highlight epmedin C as a natural caspase-1 inhibitor that mitigates DON-induced immune damage, offering a promising strategy for mycotoxin detoxification in poultry.
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