文献类型： 外文期刊

作者： Wang, Jiankui ¹ ; Hua, Guoying ¹ ; Chen, Jianfei ¹ ; Cui, Kai ¹ ; Yang, Zu ¹ ; Han, Deping ¹ ; Yang, Xue ¹ ; Dong, Xianggui ¹ ; Ma, Yuhao ¹ ; Cai, Ganxian ¹ ; Zhang, Yuanyuan ¹ ; Li, Jinnan ¹ ; Tai, Yurong ¹ ; Da, Lai ⁵ ; Li, Xinhai ⁷ ; Ma, Lina ⁸ ; Ma, Qing ⁸ ; Li, Rui ⁶ ; Liu, Jianbin ⁹ ; Darwish, Hesham Y. A. ¹⁰ ; Wu, Keliang ¹ ; Rong, Weiheng ⁵ ; Liu, Wansheng ¹¹ ; Zhao, Yaofeng ¹ ; Deng, Xuemei ¹ ;

作者机构： 1.China Agr Univ, Beijing Key Lab Anim Genet Improvement, Minist Agr, Beijing 100193, Peoples R China

2.China Agr Univ, Key Lab Anim Genet Breeding & Reprod, Minist Agr, Beijing 100193, Peoples R China

3.China Agr Univ, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China

4.Chinese Acad Agr Sci, Feed Res Inst, Beijing 100086, Peoples R China

5.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

6.Jinfeng Anim Husb Grp Co Ltd, Chifeng 024000, Peoples R China

7.Ningxia Univ, Coll Agr, Yinchuan 750021, Ningxia, Peoples R China

8.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750002, Ningxia, Peoples R China

9.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Peoples R China

10.Assiut Univ, Mol Biol Res & Studies Inst, Dept Appl Biotechnol, Assiut 71526, Egypt

11.Penn State Univ, Coll Agr Sci, Ctr Reprod Biol & Hlth, Dept Anim Sci, University Pk, PA 16802 USA

关键词： Imprinted gene; Gtl2; PI3K; AKT; mTOR-ROS signaling; REDOX imbalance; Ancestral-like wool; Primary hair follicle

期刊名称：CELL AND BIOSCIENCE （ 影响因子：7.5； 五年影响因子：7.9 ）

ISSN：

年卷期： 2023 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Background It is not uncommon for some individuals to retain certain primitive characteristics even after domestication or long-term intensive selection. Wild ancestors or original varieties of animals typically possess strong adaptability to environmental preservation, a trait that is often lacking in highly artificially selected populations. In the case of the Merino population, a world-renowned fine wool sheep breed, a phenotype with primitive coarse wool characteristic has re-emerged. It is currently unclear whether this characteristic is detrimental to the production of fine wool or whether it is linked to the adaptability of sheep. The underlying genetic/epigenetic mechanisms behind this trait are also poorly understood. Results This study identified lambs with an ancestral-like coarse (ALC) wool type that emerged during the purebred breeding of Merino fine wool sheep. The presence of this primitive sheep characteristic resulted in better environmental adaptability in lambs, as well as improved fine wool yield in adulthood. Reciprocal cross experiments revealed that the ALC phenotype exhibited maternal genetic characteristics. Transcriptomic SNP analysis indicated that the ALC phenotype was localized to the imprinted Gtl2-miRNAs locus, and a significant correlation was found between the ALC wool type and a newly identified short Interstitial Telomeric Sequences (s-ITSs) at this locus. We further confirmed that a novel 38-nt small RNA transcribed from these s-ITSs, in combination with the previously reported 22-nt small RNAs cluster from the Gtl2-miRNAs locus, synergistically inhibited PI3K/AKT/Metabolic/Oxidative stress and subsequent apoptotic pathways in wool follicle stem cells, resulting in the ALC wool type. The necessity of Gtl2-miRNAs in controlling primary hair follicle morphogenesis, as well as the wool follicle type for ALC wool lambs, was verified using intergenic differentially methylated region-knockout mice. Conclusion The ALC wool type of Merino sheep, which does not reduce wool quality but increases yield and adaptability, is regulated by epigenetic mechanisms in the imprinted Gtl2-miRNAs region on sheep chromosome 18, with the maternally expressed imprinted gene responsible for the ALC phenotype. This study highlights the significance of epigenetic regulation during embryonic and juvenile stages and emphasizes the advantages of early adaptation breeding for maternal parents in enhancing the overall performance of their offspring.