Ultrasensitive electrochemiluminescence aptasensor based on ABEI reduced silver nanoparticles for the detection of profenofos
文献类型： 外文期刊
作者： Han, Jie 1 ; Yu, Yanyang 1 ; Wang, Guanjie 1 ; Gao, Xiaolin 1 ; Geng, Lingjun 1 ; Sun, Jiashuai 1 ; Zhang, Mei 1 ; Meng, Xiaoya 1 ; Li, Falan 1 ; Shi, Ce 4 ; Sun, Xia 1 ; Guo, Yemin 1 ; Ahmed, Mohamed Bedair Mohamed 5 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Informat Technol Res Ctr, Beijing 100097, Peoples R China
5.Natl Res Ctr, Food Toxicol & Contaminants Dept, Div Food Ind & Nutr, 33 El Bohouth St, Cairo 12622, Egypt
关键词： Aptasensor; Electrochemiluminescence; ABEI-AgNPs-GO; Profenofos; Vegetables
期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）
ISSN： 0048-9697
年卷期： 2022 年 844 卷
页码：
收录情况： SCI
摘要： An ultrasensitive electrochemiluminescence (ECL) aptasensor for detection of profenofos was constructed by the reducibility and chemiluminescence property of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). ABEI was used to reduce silver nitrate (AgNO3) to silver nanoparticles (AgNPs), which could be adsorbed on the lattice of graphene oxide (GO) to form ABEI-AgNPs-GO complex. This compound could achieve excellent luminescence. The aptamer (Apt) modified (5 ') by sulfhydryl groups could be immobilized on AgNPs to capture profenofos. When profenofos was present, the ECL signal of the aptasensor would be weakened. To further demonstrate the successful construction of the aptasensor, cyclic voltammetry tests were performed on an electrochemical workstation and an ECL analyzer, respectively. The standard curve and specificity experiment both showed that the sensor had the advantages of low limit of detection (LOD) and good specificity. Under the optimal conditions, the aptasensor had a good linear response for profenofos in the range of 1 x 10(-1)-1 x 10(4) ng/mL. It also had a LOD of 6.7 x 10(-2) ng/mL and a correlation coefficient (R-2) of 0.9991. The aptasensor had been successfully applied to the detection of profenofos in vegetables. The recovery range of the proposed ECL aptasensor was 98 % similar to 107.4 %.
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