Precise Assembly of Multiple Antigens on Nanoparticles with Specially Designed Affinity Peptides
文献类型： 外文期刊
作者： Wang, Fangyu 1 ; Hu, Man 1 ; Li, Ning 2 ; Sun, Xuefeng 1 ; Xing, Guangxu 1 ; Zheng, Guanmin 3 ; Jin, Qianyue 1 ; Liu, Yunchao 1 ; Cui, Chenxu 2 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450000, Henan, Peoples R China
2.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450000, Henan, Peoples R China
3.Henan Univ Chinese Med, Publ Hlth & Prevent Med Teaching & Res Ctr, Zhengzhou 450000, Henan, Peoples R China
4.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China
5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
关键词： accurate antigen display; multiple antigen display; PLGA nanoparticles; GEM nanoparticles; affinity peptides
期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：10.383； 五年影响因子：10.382 ）
ISSN： 1944-8244
年卷期： 2022 年 14 卷 35 期
页码：
收录情况： SCI
摘要： Antigen proteins, assembled on nanoparticles, can be recognized by antigen-presenting cells effectively to enhance antigen immunogenicity. The ability to simultaneously display multiantigens on the same nanoparticle could have numerous applications but remained technical challenges. Here, we described a method for precise assembly of multiple antigens on nano-particles with specially designed affinity peptides. First, we designed and screened affinity peptides with high affinity and specificity, which could respectively target the key amino acid residues of classical swine fever virus (CSFV) E2 protein or porcine circovirus type 2 capsid protein (PCV2 Cap) accurately. Then, we conjugated the antigen proteins to poly(lactic acid- glycolic acid) copolymer (PLGA) and Gram-positive enhancer matrix (GEM) nanoparticles through the peptides and perfectly assembled two kinds of multiantigen display nanoparticles with different particle sizes. Subsequently, the immunological properties of the assembled nanoparticles were tested. The results showed that the antigen display nanoparticles could promote the maturation, phagocytosis, and proinflammatory effects of antigen-presenting cells (APCs). Besides, compared with the antigen proteins, multiantigen display nanoparticles could induce much higher levels of antibodies and neutralizing antibodies in mice. This strategy may provide a technical support for the study of protein structure and the research and development of polyvalent vaccines.
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