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Two HSP90 genes in mandarin fish Siniperca chuatsi: identification, characterization and their specific expression profiles during embryogenesis and under stresses

文献类型： 外文期刊

作者： Wang, Peng-Fei 1 ; Zeng, Shuang 1 ; Xu, Peng 1 ; Zhou, Lei 1 ; Li, Gui-Feng 1 ;

作者机构： 1.Sun Yatsen Univ, Inst Aquat Econ Anim, Sch Life Sci, Guangzhou 510006, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, Guangzhou 510300, Guangdong, Peoples R China

3.South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China

关键词： Siniperca chuatsi; HSP90; Heat shock; Hypoxia; Aeromonas hydrophila

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2016 年 42 卷 4 期

页码：

收录情况： SCI

摘要： HSP90 plays important roles in multiple cellular stress responses. Here, two cytoplasmic HSP90 isoforms, ScHSP90 alpha and ScHSP90 beta, were identified from Siniperca chuatsi. Their cDNA and gDNA structures, amino acid sequence features, and sequence identities and phylogenetic analysis with other species were described. Their expression profiles during embryonic development in different tissues and under stressful conditions were analyzed using real-time quantitative PCR. During embryogenesis, transcripts of both genes were detected at low levels during the early developmental stages and were up-regulated from appearance of myomere for ScHSP90a and closure of blastopore for ScHSP90 beta. ScHSP90 alpha showed a tissue-specific variation with high expression in ovary and brain under non-stressed conditions, while ScHSP90 beta was ubiquitously highly expressed in different tissues. Acute heat shock resulted in a strong up-regulation of ScHSP90 alpha in heart, liver, and head kidney, while it only weakly induced ScHSP90 beta in these tissues. ScHSP90 alpha was also markedly induced in liver in a time-dependent manner under hypoxia, while the expression of ScHSP90 beta was not affected by hypoxia. Additionally, Aeromonas hydrophila infection markedly augmented ScHSP90 alpha in head kidney and spleen and mildly up-regulated ScHSP90 beta in spleen, while suppressing ScHSP90 beta in head kidney. These results suggest that ScHSP90 alpha and ScHSP90 beta are differently involved in embryogenesis and under different environmental conditions including high temperature, hypoxia, and bacterial infection. This study will benefit to further clarify the roles of fish HSP90 isoforms in embryogenesis and under stressful conditions and contribute to further study on enhancing stress tolerance and disease resistance of mandarin fish.

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