Glyoxalase 1 gene of Coilia nasus: molecular characterization and differential expression during transport stress
文献类型： 外文期刊
作者： Du, Fukuan 1 ; Xu, Gangchun 2 ; Li, Yan 2 ; Nie, Zhijuan 2 ; Xu, Pao 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China
关键词： Coilia nasus; Glyoxalase 1 gene; Gene expression
期刊名称：FISHERIES SCIENCE （ 影响因子：1.617； 五年影响因子：1.531 ）
ISSN： 0919-9268
年卷期： 2016 年 82 卷 5 期
页码：
收录情况： SCI
摘要： The estuarine tapertail anchovy Coilia nasus is a widely distributed and commercially important aquaculture species. It responds strongly to stresses, such as netting, loading, and transport, which inevitably always induces tissue injury or even death. The glyoxalase 1 gene (Glo1) is very important in many physiological processes and diseases. To understand how transport induces changes in Glo1 expression, we cloned the C. nasus Glo1 gene (CnGlo1). Its full-length cDNA is 1033 bp, with a 549-bp open reading frame, which encodes a 182-amino acid protein. CnGlo1 is predicted to contain a typical glyoxalase domain (amino acids 26-169). CnGlo1 mRNA is expressed ubiquitously, but more strongly in the brain, liver, heart, head kidney, and gill than in the kidney, intestine, muscle, and spleen. Results of the reverse transcription-quantitative PCR analysis of the tissues of stressed fish revealed a 2.5- to 16.1-fold increase in CnGlo1 expression in the liver and a 2.0- to 4.8-fold increase in the brain. Protein expression was determined with western blotting, and the expression pattern was similar to that of the mRNA. Here, we report the molecular cloning, sequencing, and differential expression of the CnGlo1 gene and the effects of stress on CnGlo1 expression.
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Coilia nasus
