PcToll3 was involved in anti-Vibrio response by regulating the expression of antimicrobial peptides in red swamp crayfish, Procambarus clarkii
文献类型： 外文期刊
作者： Lan, Jiang-Feng 1 ; Wei, Shun 1 ; Wang, Yu-Qing 1 ; Dai, Yun-Jia 1 ; Tu, Jia-Gang 1 ; Zhao, Li-Juan 1 ; Li, Xin-Cang 2 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Res Ctr Marine Biol, Dept Aquat Anim Med, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
3.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China
4.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Guangdong, Peoples R China
关键词： Red swamp crayfish; PcToll3; Antimicrobial peptides; PcDorsal
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2016 年 57 卷
页码：
收录情况： SCI
摘要： Tolls and Toll-like receptors (TLRs) play an important role in host immune defenses by regulating the expression of antimicrobial peptides (AMPs) and cytokines, but the functional differences of crustacean Tolls from Drosophila Tolls or Mammal TLRs are largely unknown. A novel Toll receptor, named PcToll3, was identified from red swamp crayfish, Procambarus clarkii. It was widely expressed in all detected tissues, and its transcript in hemocytes was up-regulated at 12 h after Vibrio parahemolyticus (Vibrio) injection or at 24 h post white spot syndrome virus (WSSV) challenge. After knockdown of PcToll3, the activity of bacterial clearance was inhibited, and the expression levels of AMPs including Crustin1 (Cru1), Anti-lippopolysaccharide factor 1 (ALF1), and Lysozymes1 (Lys1), which could be up-regulated by Vibrio, were all affected. Meanwhile, PcToll3 silencing influenced the expression of myeloid differentiation factor 88 (PcMyd88), tumor necrosis factor-associated factor 6 (PcTRAF6), and PcDorsal, which were the counterparts of Drosophila Toll signaling pathway. Interestingly, PcToll3 silencing inhibited translocation of PcDorsal from cytoplasm to nucleus. Furthermore, the knockdown of PcDorsal also impaired the expression of AMPs after Vibrio challenge. Hence, we concluded that, besides participating in antiviral immunity, PcToll3 might also regulate the expression of Cru1 and Lysl to participate in anti-Vibrio immune responses by promoting PcDorsal translocation into nucleus. (C) 2016 Elsevier Ltd. All rights reserved.
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