您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A C-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish, Sebastes schlegelii

文献类型： 外文期刊

作者： Liu, Yong 1 ; Li, Ning-qiu 2 ; Zhao, Xin-peng 1 ; Yue, Bin 1 ; He, Shu-wen 1 ; Gao, Zhi-xin 1 ; Zhou, Shun 1 ; Zhang, Min 1 ;

作者机构： 1.Qingdao Agr Univ, Marine Sci & Engn Coll, 700 Changcheng Rd, Qingdao 266109, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Beijing, Peoples R China

关键词： Sebastes schlegelii; C-type lectin; Bacterial agglutination; Antibacterial; Facilitate viral infection

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2016 年 57 卷

页码：

收录情况： SCI

摘要： C-type lectins (CTLs) are important pattern recognition receptors (PRRs) that play vital roles in innate immunity. In teleosts, a number of CTLs have been reported, but their in vivo effects on host defense are still limited. In this study, a CTL homolog (SsLec1) was identified from black rockfish, Sebastes schlegelii, and its structure, expression and biological function was analyzed. The open reading frame of SsLec1 is 633 bp, with a 5'-untranslated region (UTR) of 36 bp and a 3'-UTR of 117 bp. The deduced amino acid sequence of SsLecl shares the highest overall identity (73.20%) with the CIL of Oplegnathus fasciatus. SsLecl possesses conserved CTL features, including a carbohydrate-recognition domain, four disulfide bond-forming cysteine residues, the mannose-type carbohydrate-binding motif, the conserved calcium binding sites and a putative signal peptide. The expression of SsLecl was highest in liver and could be induced by experimental infection with Listonella anguillarum. Recombinant SsLecl (rSsLecl) purified from E. coli was able to bind and agglutinate the Grain-negative fish pathogens Vibrio ichthyoenteri and Vibrio vulnificus. The agglutinating ability of rSsLecl was abolished in the presence of mannose or ethylenediaminetetraacetic acid. Further analysis showed that rSsLec1 could enhance phagocytosis by macrophages. In vivo experiments indicated that rSsLecl could inhibit bacterial infection and promote viral invasion. Taken together, these results suggest that SsLecl is a novel CTL that possesses apparent immunoregulation property and plays a critical role in host defense against pathogens invasion. (C) 2016 Elsevier Ltd. All rights reserved.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台