文献类型： 外文期刊
作者： Liu, Changlin 1 ; Zhao, Fazhen 1 ; Yan, Jingping 1 ; Liu, Chunsheng 1 ; Liu, Siwei 1 ; Chen, Siqing 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
关键词： cuttlefish; Sepia esculenta Hoyle; transcriptome sequencing; digital gene expression; early embryonic development
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2016 年 17 卷 10 期
页码：
收录情况： SCI
摘要： Golden cuttlefish Sepia esculenta Hoyle is an economically important cephalopod species. However, artificial hatching is currently challenged by low survival rate of larvae due to abnormal embryonic development. Dissecting the genetic foundation and regulatory mechanisms in embryonic development requires genomic background knowledge. Therefore, we carried out a transcriptome sequencing on Sepia embryos and larvae via mRNA-Seq. 32,597,241 raw reads were filtered and assembled into 98,615 unigenes (N50 length at 911 bp) which were annotated in NR database, GO and KEGG databases respectively. Digital gene expression analysis was carried out on cleavage stage embryos, healthy larvae and malformed larvae. Unigenes functioning in cell proliferation exhibited higher transcriptional levels at cleavage stage while those related to animal disease and organ development showed increased transcription in malformed larvae. Homologs of key genes in regulatory pathways related to early development of animals were identified in Sepia. Most of them exhibit higher transcriptional levels in cleavage stage than larvae, suggesting their potential roles in embryonic development of Sepia. The de novo assembly of Sepia transcriptome is fundamental genetic background for further exploration in Sepia research. Our demonstration on the transcriptional variations of genes in three developmental stages will provide new perspectives in understanding the molecular mechanisms in early embryonic development of cuttlefish.
- 相关文献
作者其他论文 更多>>
-
Chrome-Free Tanning Process of Thamnaconus septentrionalis Skin
作者：Pan, Luying;Yang, Yun;Kong, Qing;Pan, Luying;Yang, Yun;Bian, Li;Chen, Siqing;Kong, Qing
关键词：Thamnaconus septentrionalis; Fish skin waste utilization; Chrome-free tanning; Fish leather; Application
-
Near complete genome assembly of Yadong trout (Salmo trutta)
作者：Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Li, Chen;Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Shao, Changwei
关键词：
-
Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
作者：Zheng, Weiwei;Liu, Changlin;Han, Shenglei;Yang, Tao;Liu, Zhihong;Xu, Dong;Shao, Changwei;Liu, Kaiqiang;Zheng, Weiwei;Liu, Changlin;Liu, Zhihong;Xu, Dong;Shao, Changwei;Liu, Kaiqiang;Wang, Tengteng;Han, Huizong;Xi, Xiaoqing
关键词：
-
Effects of alanine supplementation on the growth, biochemical composition and appetite of ivory shell (Babylonia areolata)
作者：Hong, Xin;Sun, Yunchao;Liu, Jingcan;Yu, Feng;Yang, Yi;Liu, Chunsheng;Sun, Yunchao;Yu, Feng;Yang, Yi;Liu, Chunsheng
关键词：Babylonia areolata; Alanine supplementation; Growth performance; Amino acid composition; Appetite
-
Gonadal Transcriptome Sequencing Analysis Reveals the Candidate Sex-Related Genes and Signaling Pathways in the East Asian Common Octopus, Octopus sinensis
作者：Li, Fenghui;Chen, Siqing;Pan, Luying;Liu, Changlin;Bian, Li;Li, Fenghui;Chen, Siqing;Pan, Luying;Liu, Changlin;Bian, Li;Zhang, Tao
关键词：Octopus sinensis; gonadal transcriptome sequence; gene-expression profile; sex-related genes and pathways
-
Transcriptome Analysis of Juvenile Black Rockfish Sebastes schlegelii under Air Exposure Stress
作者：Liu, Changlin;Zhang, Zheng;Wei, Shouyong;Xiao, Wenjie;Xiao, Wenjie;Wang, Yue;Zhao, Chao;Yang, Liguo;Yang, Liguo
关键词：Sebastes schlegelii; transcriptome sequencing; air exposure; stress
-
Genome-Wide Identification, Molecular Characterization, and Expression Analysis of the HSP70 and HSP90 Gene Families in Thamnaconus septentrionalis
作者：Chen, Ying;Chen, Ying;Chang, Qing;Zhang, Ziyang;Wu, Dan;Bian, Li;Chen, Siqing;Fang, Qinmei
关键词：Thamnaconus septentrionalis; HSP70; HSP90; tissue; developmental stage; abiotic stress
