Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)
文献类型： 外文期刊
作者： Wang, Yeda 1 ; Li, Zeming 1 ; Lu, Yuanan 4 ; Hu, Guangfu 1 ; Lin, Li 1 ; Zeng, Lingbing 5 ; Zhou, Yong 5 ; Liu, Xueqin 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
3.Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
4.Univ Hawaii Manoa, Dept Publ Hlth Sci, Honolulu, HI 96822 USA
5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Peoples R China
关键词： TRIM32; spring viraemia of carp virus; common carp
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2016 年 17 卷 10 期
页码：
收录情况： SCI
摘要： Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro.
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