A New Microbial Biosensor for Detecting and Monitoring Water Acute Toxicity Based on Methylene Blue
文献类型： 外文期刊
作者： Wu Li-Dong 1 ; Liu Ling 2 ; Li Dan 2 ; Fu Xiao-Chen 1 ; Liu Huan 1 ; Song Yi 1 ; Ma Bing 1 ; Zhai Jun-Feng 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Beijing 344000, Peoples R China
2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
关键词： Acute water toxicity; Methylene blue; Intact cell; Damaged cell; P. fluorescens
期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）
ISSN： 0253-3820
年卷期： 2016 年 44 卷 9 期
页码：
收录情况： SCI
摘要： A novel method based on methylene blue (MB) was established for the rapid determination of acute water toxicity with the P. fluorescens as the test organism and the damage of cell membrane as the toxicity response. In this method, the damaged rather than intact cells could be stained positively by MB. Therefore, in the presence of toxicants that could induce the damage of cell membrane, MB would enter into cells, resulting in a decrease in MB concentration of the solution. The acute water toxicity could thus be determined by measuring the changes optically in the concentration of MB in the solutions. The present results displayed that, the detection limits of the reported method for 3,5-dichlorophenol, As3+ and Hg2+ were 1. 6, 12. 5 and 3.2 mg/L, respectively. In special, the detection limit of the reported method for 3,5. dichlorophenol (DCP) was lower than that of the commercial equipment, Baroxymeter. The reported method is simple, rapid and reliable, and shows an excellent promise in the determination and monitoring of acute toxicity.
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