文献类型： 外文期刊

作者： Dong, Chuanju ¹ ; Chen, Lin ¹ ; Feng, Jingyan ² ; Xu, Jian ⁴ ; Mahboob, Shahid ⁶ ; Al-Ghanim, Khalid ¹ ; Li, Xuejun ¹ ; X ¹ ;

作者机构： 1.Henan Normal Univ, Coll Fishery, Xinxiang, Henan, Peoples R China

2.Shanghai Ocean Univ, Coll Life Sci, Shanghai, Peoples R China

3.Xiamen Univ, Coll Ocean & Earth Sci, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Xiamen, Peoples R China

4.Chinese Acad Fishery Sci, Key Lab Aquat Genom, Beijing, Peoples R China

5.Chinese Acad Fishery Sci, Beijing Key Lab Fishery Biotechnol, Ctr Appl Aquat Genom, Beijing, Peoples R China

6.King Saud Univ, Coll Sci, Dept Zool, Riyadh, Saudi Arabia

7.GC Univ, Dept Zool, Faisalabad, Pakistan

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2016 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Background Aquaporins (Aqps) are integral membrane proteins that facilitate the transport of water and small solutes across cell membranes. Among vertebrate species, Aqps are highly conserved in both gene structure and amino acid sequence. These proteins are vital for maintaining water homeostasis in living organisms, especially for aquatic animals such as teleost fish. Studies on teleost Aqps are mainly limited to several model species with diploid genomes. Common carp, which has a tetraploidized genome, is one of the most common aquaculture species being adapted to a wide range of aquatic environments. The complete common carp genome has recently been released, providing us the possibility for gene evolution of aqp gene family after whole genome duplication. Results In this study, we identified a total of 37 aqp genes from common carp genome. Phylogenetic analysis revealed that most of aqps are highly conserved. Comparative analysis was performed across five typical vertebrate genomes. We found that almost all of the aqp genes in common carp were duplicated in the evolution of the gene family. We postulated that the expansion of the aqp gene family in common carp was the result of an additional whole genome duplication event and that the aqp gene family in other teleosts has been lost in their evolution history with the reason that the functions of genes are redundant and conservation. Expression patterns were assessed in various tissues, including brain, heart, spleen, liver, intestine, gill, muscle, and skin, which demonstrated the comprehensive expression profiles of aqp genes in the tetraploidized genome. Significant gene expression divergences have been observed, revealing substantial expression divergences or functional divergences in those duplicated aqp genes post the latest WGD event. Conclusions To some extent, the gene families are also considered as a unique source for evolutionary studies. Moreover, the whole set of common carp aqp gene family provides an essential genomic resource for future biochemical, toxicological, physiological, and evolutionary studies in common carp.