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Identification, characterization and expression analysis of tumor suppressor protein p53 from pufferfish (Takifugu obscurus) after the Vibrio alginolyticus challenge

文献类型： 外文期刊

作者： Cheng, Chang-Hong 1 ; Luo, Sheng-Wei 2 ; Ye, Chao-Xia 2 ; Wang, An-Li 2 ; Guo, Zhi-Xun 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr,Key Lab Aquat Product Proc Key Lab Fis, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

2.South China Normal Univ, Sch Life Sci, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangdong Prov Key Lab Healthy & Safe Aquaculture, Guangzhou 510300, Guangdong, Peoples R China

关键词： Takifugu obscurus; p53; Gene expression; Vibrio alginolyticus; Apoptosis

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2016 年 59 卷

页码：

收录情况： SCI

摘要： The tumor suppressor protein p53 plays a critical role in cell cycle, apoptosis and DNA repair. In this study, the full-length pufferfish p53 (Pf-p53) was obtained, containing an open reading frame of 1095 bp, a 5'UTR of 157 bp and a 3'UTR of 285 bp with a poly (A) tail. The Pf-p53 encoded a polypeptide of 364 amino acids with a theoretical isoelectric point of 8.03 and predicted molecular weight of 40.6 kDa. Pfp53 was ubiquitously expressed in various tissues with a high-level expression in kidney, liver and gill. Vibrio alginolyticus challenge could induce ROS production and disrupt Ca2+ homeostasis, subsequently leading to the induction of DNA damage and apoptosis, while the Vibrio alginolyticus-induced oxidative stress can also increase the non-specific immunity. The pufferfish challenged with Vibrio alginolyticus showed a sharp increase of Pf-p53 transcript in liver. Subcellular localization analysis revealed that Pfp53 was primarily localized in nucleus. Furthermore, overexpression of Pf-p53 in Hela cells could inhibit cell proliferation and the transcriptional activities of the NF-kappa B promoter. Taken together, our results indicated that Pf-p53 may play an important role in the immune response to Vibrio alginolyticus challenge. (C) 2016 Elsevier Ltd. All rights reserved.

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