Identification of peptides from sinus gland extracts in the red swamp crayfish Procambarus clarkii by mass spectrometry
文献类型: 外文期刊
作者: Shui, Yan 1 ; Xu, Zeng-Hong 1 ; Zhou, Xin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
关键词: Electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS; Neuropeptides; Procambatrus clarkia; Sinus gland (SG)
期刊名称:INDIAN JOURNAL OF ANIMAL RESEARCH ( 影响因子:0.44; 五年影响因子:0.543 )
ISSN: 0367-6722
年卷期: 2016 年 50 卷 6 期
页码:
收录情况: SCI
摘要: Crustacean sinus gland (SG) is a well-defined neuroendocrine organ that controls the secretion of various neuropeptides which regulate many physiological activities. The red swamp crayfish Procambarus clarkii is a decapod crustacean with both high economic and scientific importance in China. To facilitate physiological investigations of SG peptide/hormone function in this species, we have been employed tissue extract fractionation by HPLC to obtain a complete description of neuropeptidome and used Electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS) to detect the peptidome present in its organ. In total, 48 peptide sequences belonging to several known neuropeptide families including crustacean cardioactive peptide, CHH precursor-related peptides (CPRPs), orcokinins and pigment dispersing hormones were identified. Among these 48 sequences, 26 are novel peptides and 22 are previously identified. Overall, the results give a stimulus for future physiological studies of SG neuropeptides in P. clarkii and other crustaceans.
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