Development and Characterization of Microsatellite Markers for the Ornamented Pygmy Frog Microhyla fissipes and Transferability in Microhyla
文献类型： 外文期刊
作者： Liu, Lusha 1 ; Cheng, Lei 2 ; Zhang, Xingqi 3 ; Li, Yulong 1 ; Jiang, Jianping 1 ;
作者机构： 1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Haerbin 150076, Peoples R China
3.Sichuan Normal Univ, Chengdu 610101, Sichuan, Peoples R China
关键词： Microhyla fissipes; Genic microsatellites; Transcriptome; Transferability; Microhyla
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2019 年 51 卷 5 期
页码：
收录情况： SCI
摘要： Microhyla fissipes, from family Microhylidae suborder Neobatrachia, widely distributes from western Myanmar eastward through Indochina and northward into southern China including Hainan and Taiwan. But the availability of microsatellites especially from expressed sequences is currently very limited in this species and the genus Microhyla. A total of 17,339 potential microsatellites were identified in 15,385 unigenes which were generated by Illumina paired-end sequencing in M. fissipes. Within all microsatellites, AG/CT, AAG/CTT, and AAAG/CTTT are most prevalent motif in each repeat class. We randomly selected 61 unigenes with the microsatellite to design primers and do genetic analysis in the Sichuan basin population of M. fissipes. Of all, 35 primer pairs (57.38%) successfully amplificated in M. fissipes, of which 14 (40.00%) were polymorphism. The observed and expected heterozygosity in the test population ranged from 0.02 to 0.92 and from 0.02 to 0.62, respectively. High transferability rates were detected in M. butleri (37.14%), M. heymonsi (45.71%), M. pulchra (57.14%) and M. mixture (71.43%). These results indicate that the Illumina paired-end sequencing system is of great value for identifying massive numbers of genic microsatellites in M. fissipes with high-efficiency. Furthermore, the described polymorphic loci in this study should be useful for population genetic and conservation genetic studies in M. fissipes and other closely related species from this important genus Microhyla.
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