Comprehensive identification and profiling of Chinese shrimp (Fenneropenaeus chinensis) microRNAs in response to high pH stress using Hiseq2000 sequencing
文献类型： 外文期刊
作者： Li, Zhaoxia 1 ; Wang, Jinye 1 ; He, Yuying 2 ; Hu, Shuo 4 ; Wang, Qingyin 2 ; Li, Jian 2 ;
作者机构： 1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
关键词： Fenneropenaeus chinensis; high pH stress; Hiseq sequencing; microRNA
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期： 2019 年 50 卷 11 期
页码：
收录情况： SCI
摘要： pH has a great impact on the distribution, growth, behaviour and physiology in many aquatic animals. MicroRNAs (miRNAs), a class of small non-coding RNAs play essential roles in animal responses to environmental stress. In this study, we constructed two small RNA (sRNA) libraries from control (pH 7.4) and high pH (9.4) treated shrimp to identify high pH stress-responsive miRNAs. Using deep sequencing, we identified 72 (3 conserved and 69 novel) up- and 4 (2 conserved and 2 novel) down-regulated miRNAs from high pH challenged shrimp hepatopancreas. Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that these target transcripts were implicated in a broad range of biological processes and various metabolic pathways. Moreover, we validated the expression patterns of selected miRNAs and their target genes by qRT-PCR. The present work adds new insights into the large-scale identification and characterization of Fenneropenaeus chinensis miRNAs and their potential targets and represents a foundation for underlying mechanisms of animal responses to high pH stress at post-transcriptional level.
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