Physiological Adaptation of Fenneropenaeus chinensis in Response to Saline-Alkaline Stress Revealed by a Combined Proteomics and Metabolomics Method
文献类型： 外文期刊
作者： Gao, Tian 1 ; Wang, Qiong 2 ; Sun, Huarui 1 ; Liu, Yang 1 ; Li, Jitao 2 ; He, Yuying 2 ;
作者机构： 1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Natl Key Lab Mariculture Biobreeding & Sustainable, Qingdao 266071, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266200, Peoples R China
关键词： Fenneropenaeus chinensis; proteomics; metabolomics; carbonate alkalinity stress; high pH stress
期刊名称：BIOLOGY-BASEL （ 影响因子：3.6； 五年影响因子：3.8 ）
ISSN：
年卷期： 2024 年 13 卷 7 期
页码：
收录情况： SCI
摘要： The rapid development of the mariculture industry has been hindered by limited coastal aquaculture space. To utilize the abundant inland saline-alkaline water, we studied the physiological effects of high carbonate alkalinity stress and high pH stress on Fenneropenaeus chinensis. The study employed quantitative proteomics by tandem mass tag (TMT) and non-targeted metabolomics analysis using a liquid chromatograph mass spectrometer (LC-MS) to understand the physiological and biochemical adaptive mechanisms of the hepatopancreas of F. chinensis in response to saline-alkaline stress at the molecular level. We designed two stress groups as follows: a high carbonate alkalinity (CA) group and a combined high carbonate alkalinity and high pH (CP) group. The study found that the protein and metabolic profiles of the two stress groups were changed, and the CP group, which was exposed to dual stresses, incurred more severe damage to the hepatopancreas compared to that of the CA group. After exposure to CA and CP, the hepatopancreas of F. chinensis showed significant alterations in 455 proteins and 50 metabolites, and 1988 proteins and 272 metabolites, respectively. In addition, F. chinensis upregulated the level of energy metabolism in the hepatopancreas to defend against osmotic imbalance caused by CA or CP stress, which was demonstrated by the significant upregulation of important proteins and metabolites in glycolysis, pyruvate metabolism, TCA cycle, and fatty acid oxidation. Additionally, pattern recognition receptors, the phenol oxidase system, and various immune-related metabolic enzymes and metabolites were also affected. The immune homeostasis of F. chinensis was affected by the alteration of the antioxidant system following exposure to CA or CP. These findings provide valuable information for F. chinensis saline-alkaline water cultivation practices.
- 相关文献
作者其他论文 更多>>
-
Comparative Transcriptome Analysis of the Hypothalamic-Pituitary-Gonadal Axis of Jinhu Grouper (Epinephelus fuscoguttatus ♀ x Epinephelus tukula ♂) and Tiger Grouper (Epinephelus fuscoguttatus)
作者：Qiu, Yishu;Duan, Pengfei;Ding, Xiaoyu;Li, Zhentong;Wang, Xinyi;Li, Linlin;Liu, Yang;Wang, Linna;Tian, Yongsheng;Li, Zhentong;Li, Linlin;Liu, Yang;Wang, Linna;Tian, Yongsheng;Li, Zhentong;Li, Linlin;Liu, Yang;Wang, Linna;Tian, Yongsheng
关键词：Jinhu grouper (Epinephelus fuscoguttatus female x Epinephelus tukula male); Epinephelus fuscoguttatus; gonadal development; transcriptome
-
Surficial engineering of active hydroxyls for ambient formaldehyde oxidation via enhanced Lewis acidity over Zr-doped cryptomelane materials
作者：Wang, Hui-Jun;Yang, Huan-Huan;Chen, Tian-Yun;Zhan, Jingjing;Zhou, Hao;Yi, Xianliang;Zhang, Shi -Yu;Liu, Yang;Li, Zhonghong;Shen, Xudong
关键词：Air purification; Formaldehyde; Catalytic oxidation; Manganese oxide; Hydroxyl; Structure; Performance relation
-
Genetic structural analysis of different breeds and geographical groups of Fenneropenaeus chinensis reveals population diversity
作者：Wang, Qiong;Jiang, Yuhan;Li, Jian;Li, Jitao;He, Yuying;Wang, Qiong;Li, Jian;Li, Jitao;He, Yuying
关键词：Genetic structure; Fenneropenaeus chinensis; Population; Commercial breed; Selection signature
-
Genome-wide identification of ATG genes and their expression profiles under biotic and abiotic stresses in Fenneropenaeus chinensis
作者：Guan, Chenhui;Li, Yalun;Li, Zhaoxia;Guan, Chenhui;Li, Yalun;Wang, Qiong;Wang, Jiajia;Tian, Caijuan;He, Yuying;Wang, Qiong;Wang, Jiajia;He, Yuying;Tian, Caijuan
关键词：Fenneropenaeus chinensis; Autophagy; WSSV; Low-salt stress
-
Effects of Environmental Hypoxia on Serum Hematological and Biochemical Parameters, Hypoxia-Inducible Factor (hif) Gene Expression and HIF Pathway in Hybrid Sturgeon (Acipenser schrenckii ♂ x Acipenser baerii ♀)
作者：Ren, Yuanyuan;Cheng, Bo;Ren, Yuanyuan;Tian, Yuan;Liu, Yang;Yu, Huanhuan
关键词：hypoxia; physiological response; hypoxia-induced factor; HIF pathway; hybrid sturgeon
-
Chromosome-level genome assembly of ridgetail white shrimp Exopalaemon carinicauda
作者：Wang, Jiajia;Lv, Jianjian;Ge, Qianqian;Wang, Qiong;He, Yuying;Li, Jian;Li, Jitao;Wang, Jiajia;Lv, Jianjian;Ge, Qianqian;Wang, Qiong;He, Yuying;Li, Jian;Li, Jitao;Shi, Miao
关键词：
-
Dietary Lysophosphatidylcholine Improves the Uptake of Astaxanthin and Modulates Cholesterol Transport in Pacific White Shrimp Litopenaeus vannamei
作者：Song, Ziling;Liu, Yang;Song, Ziling;Liu, Yang;Liu, Huan;Ye, Zhengwei;Ma, Qiang;Wei, Yuliang;Liang, Mengqing;Xu, Houguo;Xiao, Lindong
关键词：carotenoid; lysophospholipid; lipid metabolism; antioxidant capacity; shrimp quality
