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Expressional analysis of the silkworm storage protein 1 and identification of its interacting proteins

文献类型： 外文期刊

作者： Li, Ruilin 1 ; Hu, Congwu 1 ; Geng, Tao 3 ; Lv, Dingding 4 ; Gao, Kun 1 ; Guo, Xijie 1 ; Hou, Chengxiang 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang, Jiangsu, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Res Inst, Haikou, Hainan, Peoples R China

4.Zhenjiang Coll, Zhenjiang, Jiangsu, Peoples R China

关键词： Bombyx mori; BmSP1; antimelanization; BmLP6; interacting protein

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN： 0962-1075

年卷期：

页码：

收录情况： SCI

摘要： Storage proteins are haemolymph-specific proteins in insects, mainly synthesized in the fat body, released into the haemolymph, and then selectively reabsorbed by the fat body before pupation. These storage proteins play an important role in insect metamorphosis and egg development. Some of these storage proteins are responsive to pathogen infection and can even suppress pathogen multiplication. However, the mechanisms of the physiological, biochemical and immune-responsive functions of storage proteins remain unclear. In this study, the expression patterns of Bombyx mori storage protein 1 (BmSP1) during the larval stage were analysed. Then, BmSP1 protein fused with enhanced green fluorescent protein (EGFP) was successfully expressed in a B. mori baculovirus vector expression system. Quantitative real-time PCR showed that the expression level of BmSP1 increased with the advance of instars and reached the highest level in the fifth instar, especially in the fat body. Recombinant BmSP1 expressed in silkworm larvae inhibited haemolymph melanization. Then, proteins that interact with BmSP1 were identified with EGFP used as an antigenic determinant by co-immunoprecipitation. A 30 kDa low molecular weight lipoprotein PBMHP-6 precursor (BmLP6) was shown to interact with BmSP1. Yeast two-hybrid experiments confirmed the interaction between BmSP1 and BmLP6. The results obtained in this study will be helpful for further study of the functions of BmSP1 and BmLP6 in the regulatory network of silkworm development and innate immunity.

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