文献类型： 外文期刊

作者： Wu, Xia ¹ ; Chi, Xiaoyan ¹ ; Wang, Yanhua ¹ ; Zhang, Kailu ¹ ; Kai, Le ¹ ; He, Qiuning ¹ ; Tang, Jinxiu ¹ ; Wang, Kewen ¹ ;

作者机构： 1.Ludong Univ, Sch Life Sci, Dept Appl & Environm Microbiol, Yantai 264025, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Urumqi 830001, Peoples R China

关键词： P. chlororaphis; phenazine-1-carboxylic acid; pyrrolnitrin; regulation; Vfr

期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）

ISSN： 1598-2254

年卷期： 2019 年 35 卷 4 期

页码：

收录情况： SCI

摘要： In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role in suppression of mycelial growth of some fungal pathogens that cause plant diseases in agriculture. Although some regulators for pyrrolnitrin biosynthesis were identified, the pyrrolnitrin regulation pathway was not fully constructed. During our screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant G05 Delta phz4prn::lacZ and E. coli S17-1 (pUT/mini-Tn5Kan). By cloning and sequencing of the transposon-flanking DNA fragment, we found that a vfr gene in the conjugant G05W02 was disrupted with mini-Tn5Kan. In one other previous study on P. fluorescens, however, it was reported that the deletion of the vfr caused increased production of pyrrolnitrin and other antifungal metabolites. To confirm its regulatory function, we constructed the vfr-knockout mutant G05 Delta vfr and G05 Delta phz Delta prn::lacZ Delta vfr. By quantifying beta-galactosidase activities, we found that deletion of the vfr decreased the prn operon expression dramatically. Meanwhile, by quantifying pyrrolnitrin production in the mutant G05 Delta vfr, we found that deficiency of the Vfr caused decreased pyrrolnitrin production. However, production of phenazine-l-carboxylic acid was same to that in the wild-type strain G05. Taken together, Vfr is required for pyrrolnitrin but not for phenazine-l-carboxylic acid biosynthesis in P. chlororaphis G05.