文献类型： 外文期刊

作者： Xie, Qing ¹ ; Shen, Kang-Ning ² ; Hao, Xiuying ³ ; Phan Nhut Nam ⁴ ; Bui Thi Ngoc Hieu ⁴ ; Chen, Ching-Hung ⁶ ; Zhu, C ¹ ;

作者机构： 1.Xinjiang Normal Univ, Res Room Mol Biol & Informat, Urumqi, Peoples R China

2.Natl Taiwan Ocean Univ, Ctr Excellence Oceans, Keelung, Taiwan

3.Xinjiang Acad Agr Sci, Inst Microbiol, Ulumuqi, Peoples R China

4.Ton Duc Thang Univ, Fac Appl Sci, Ho Chi Minh City, Vietnam

5.Chung Yuan Christian Univ, Dept Biosci Technol, 200 Chung Pei Rd, Chungli 32023, Taiwan

6.WeThink Biotech Inc, Taoyuan, Taiwan

7.Chinese Culture Univ, Grad Inst Biotechnol, Taipei 11114, Taiwan

关键词： Chioroplast genome; next generation sequencing; Saussurea involucrata; Snow lotus

期刊名称：MITOCHONDRIAL DNA PART A （ 影响因子：1.514； 五年影响因子：0.707 ）

ISSN： 2470-1394

年卷期： 2017 年 28 卷 1-2 期

页码：

收录情况： SCI

摘要： We decoded the complete chloroplast DNA (cpDNA) sequence of the Tianshan Snow Lotus (Saussurea involucrata), a famous traditional Chinese medicinal plant of the family Asteraceae, by using next-generation sequencing technology. The genome consists of 152 490 bp containing a pair of inverted repeats (IRs) of 25 202 bp, which was separated by a large single-copy region and a small single-copy region of 83 446 bp and 18 639 bp, respectively. The genic regions account for 57.7% of whole cpDNA, and the GC content of the cpDNA was 37.7%. The S. involucrata cpDNA encodes 114 unigenes (82 protein-coding genes, 4 rRNA genes, and 28 tRNA genes). There are eight protein-coding genes (atpF, ndhA, ndhB, rpI2, rpoC1, rps16, dpP, and ycf3) and five tRNA genes (trnA-UGC, trnl-GAU, trnK-UUU, trnL-UAA, and trnV-UAC) containing introns. A phylogenetic analysis of the 11 complete cpDNA from Asteracease showed that S. involucrata is closely related to Centaurea diffusa (Diffuse Knapweed). The complete cpDNA of S. involucrata provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for Asteraceae.