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Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China

文献类型： 外文期刊

作者： Bai, Yan 1 ; Huang, Xiying 1 ; Zhou, Xiangrui 1 ; Xiang, Quanju 1 ; Zhao, Ke 1 ; Yu, Xiumei 1 ; Chen, Qiang 1 ; Jiang, Ha 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Resources, Dept Microbiol, Chengdu, Sichuan, Peoples R China

2.Chinese Acad Sci, Inst Mt Hazards & Environm, Chengdu, Sichuan, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Agr Resources & Environm Sci, Lhasa, Tibet, Peoples R China

关键词： Hailuogou Glacier retreat area; Primary succession; Denitrifying bacteria; Community; Abundance

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2019 年 7 卷

页码：

收录情况： SCI

摘要： Background. The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier's retreated area provides an excellent living environment for the colonization of microbes and plants, making it an ideal model to explore plant successions, microbial communities, and the interaction of plants and microbes during the colonization process. However, to date, the density of the nitrogen cycling microbial communities remain unknown, especially for denitrifiers in the primary succession of the Hailuogou Glacier. Therefore, we investigated the structural succession and its driving factors for denitrifying bacterial communities during the four successional stages (0, 20, 40, and 60 years). Methods. The diversity, community composition, and abundance of nosZ-denitrifiers were determined using molecular tools, including terminal restriction fragment length polymorphism and quantitative polymerase chain reactions (qPCR). Results. nosZ-denitrifiers were more abundant and diverse in soils from successional years 20-60 compared to 0-5 years, and was highest in Site3 (40 years). The denitrifying bacterial community composition was more complex in older soils (40-60 years) than in younger soils (<= 20 years). The terminal restriction fragments (T-RFs) of Azospirillum (90 bp) and Rubrivivax (95 bp) were dominant in soisl during early successional stages (0-20 years) and in the mature phase (40-60 years), respectively. Specific T-RFs of Bradyrhizobium (100 bp) and Pseudomonas (275 bp) were detected only in Site3 and Site4, respectively. Moreover, the unidentified 175 bp T-RFs was detected only in Site3. Of the abiotic factors that were measured in this study, soil available phosphorus, available potassium and denitrifying enzyme activity (DEA) correlated significantly with the community composition of nosZ-denitrifiers (P < 0.05 by Monte Carlo permutation test within RDA analysis).

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