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Evaluation of an ELISA-based visualization microarray chip technique for the detection of veterinary antibiotics in milk

文献类型： 外文期刊

作者： Du, Bingyao 1 ; Wen, Fang 1 ; Guo, Xiaodong 1 ; Zheng, Nan 1 ; Zhang, Yangdong 1 ; Li, Songli 1 ; Zhao, Shengguo 1 ; Liu, 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Qual & Safety Control Milk & Dairy Prod, Inst Anim Sci, Minist Agr & Rural Affairs, 2 Yuan Ming Yuan West Rd, Beijing 100193, Peoples R China

2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Gansu, Peoples R China

3.Univ Liege, Gembloux Agrobio Tech, Chim Gen & Organ, Passage Deportes 2, B-5030 Gembloux, Belgium

4.Chinese Acad Agr Sci, Lab Qual & Safety Risk Assessment Dairy Prod, Minist Agr & Rural Affairs, Inst Anim Sci, Beijing 100193, Peoples R China

5.Chinese Acad Agr Sci, Milk & Milk Prod Inspect Ctr, Minist Agr & Rural Affairs, Inst Anim Sci, Beijing 100193, Peoples R China

6.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China

7.Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China

关键词： Evaluation; ELISA; Veterinary antibiotics; Detection; Milk

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2019 年 106 卷

页码：

收录情况： SCI

摘要： Antibiotics are increasingly found in dairy products. To ensure consumer safety, rapid screening methods which are accurate and cost-effective are highly in demand. A multiplexed detection technology was tested for quantification of these four kinds of antibiotics simultaneously in milk. The visual microarray technology based on enzyme-linked immunosorbent assays was utilized in this method, which can detect multiple target molecules simultaneously. This colorimetric technology provides direct results that can be detected using common commercial microplate scanners. The limit of detection (LOD) were calculated as 3.30, 3.39, 2.42 and 4.88 mu g kg(-1) for quinolones, tetracycline, lincomycin and streptomycin, respectively. The limits of quantity (LOQ) were defined as 8.97, 8.43, 5.36 and 10.97 mu g kg(-1) for quinolones, tetracycline, lincomycin and streptomycin, respectively. The accuracy of method was also adequate for these four substances with the recovery rates ranging from 77.6% to 116.4% were obtained for the detection of practical samples. According to our evaluation, this methodology can be used for rapid screening of antibiotics in internal quality control programs in dairy industry.

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