您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Nuclease Triggered "Signal-On" and Amplified Fluorescent Sensing of Fumonisin B-1 Incorporating Graphene Oxide and Specific Aptamer

文献类型： 外文期刊

作者： Guo, Xiaodong 1 ; Qiao, Qinqin 3 ; Zhang, Mengke 1 ; Fauconnier, Marie-Laure 2 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

2.Univ Liege, Gembloux Agrobio Tech, Chim Gen & Organ, Passage Deportes 2, B-5030 Gembloux, Belgium

3.Chinese Acad Agr Sci, Lab Qual & Safety Risk Assessment Dairy Prod, Minist Agr & Rural Affairs, Inst Anim Sci, Beijing 100193, Peoples R China

4.Fuyang Normal Univ, Coll Informat Engn, Fuyang 236041, Peoples R China

关键词： aptasensor; fumonisin B-1; nuclease; graphene oxide; point-of-care testing; food safety

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 16 期

页码：

收录情况： SCI

摘要： Remarkable advancements have been achieved in the development of rapid analytic techniques toward fumonisin B-1 (FB1) monitoring and even trace levels for food safety in recent years. However, the point-of-care testing for quantitative and accurate FB1 determination is still challenging. Herein, an innovative aptasensor was established to monitor FB1 by utilizing graphene oxide (GO) and nuclease-triggered signal enhancement. GO can be utilized as a fluorescence quenching agent toward a fluorophore-modified aptamer, and even as a protectant of the aptamer from nuclease cleavage for subsequent target cycling and signal amplification detection. This proposed sensing strategy exhibited a good linearity for FB1 determination in the dynamic range from 0.5 to 20 ng mL(-1) with a good correlation of R-2 = 0.995. Its limit of detection was established at 0.15 ng mL(-1) (S/N = 3), which was significantly lower than the legal requirements by three orders of magnitude. The interferent study demonstrated that the introduced aptasensor possessed high selectivity for FB1. Moreover, the aptasensor was successfully applied to the detection of wheat flour samples, and the results were consistent with the classical ELISA method. The rapid response, sensitive and selective analysis, and reliable results of this sensing platform offer a promising opportunity for food mycotoxin control in point-of-care testing.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台