文献类型： 外文期刊

作者： Ahmad, Aqeel ¹ ; Akram, Waheed ¹ ; Shahzadi, Iqra ² ; Wang, Rui ¹ ; Hu, Du ¹ ; Bashir, Zoobia ³ ; Jaleel, Waqar ⁴ ; Ahmed ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China

2.Wuhan Univ, Sch Resource & Environm Sci, Wuchang, Peoples R China

3.Nanjing Univ, Dept Phys, Natl Lab Solid State Microstruct, Nanjing, Jiangsu, Peoples R China

4.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou, Guangdong, Peoples R China

5.Univ Austral Chile, Fac Ciencias, Inst Farm, Campus Isla Teja, Valdivia, Chile

6.Univ Punjab, Inst Agr Sci, Lahore, Pakistan

7.Tech & Further Educ, Brisbane, Qld, Australia

关键词： bioactive compounds; chemical enrichment; mass spectrum analysis; nutritional augmentation; Penicillium oxalicum metabolites; protein profiling

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.638； 五年影响因子：3.802 ）

ISSN： 0022-5142

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收录情况： SCI

摘要： BACKGROUND Tomato is an important food item and a cocktail of phytonutrients. In the current study, metabolites from a non-pathogenic fungal species Penicillium oxalicum have been exploited to obtain nutritionally augmented tomato fruits from the plants to better withstand against Alternaria alternata infection. RESULTS Initially, bioactivity-guided assay and chromatographic analyses identified the bioactive metabolites of P. oxalicum [benzenedicarboxylic acid (BDA) and benzimidazole]. Then, >= 3 times elevated quantities of vitamins and other nutritional elements (protein, fat, fibers, and carbohydrates) were achieved by the foliar application of BDA. The maximum increase (625.81%) was recorded in riboflavin contents; however, thiamine showed the second highest enhancement (542.86%). Plant metabolites analysis revealed that jasmonic acid contents were boosted 121.53% to significantly enhance guaiacyl lignin defenses along with the reduction in coumarin contents. The protein profile analysis explored three most actively responding protein species toward BDA applications, (i) palmitoyltransferase protein Q9FLM3; (ii) serine/threonine-protein kinase O48814; and (iii) E3 ubiquitin-protein ligase Q9FJQ8. The O48814 improved plant defenses; whereas, Q9FJQ8 protein was negatively regulating cysteine-type endopeptidase activity and assisted plant to resist schedule alterations. Tomato cultivar with more active innate metabolism was found to be more responsive toward BDA. Furthermore, the bioactive compounds were enriched by using the two-step extraction method of ethyl acetate and chloroform, respectively. CONCLUSION Penicillium oxalicum a non-pathogenic fungal species, produced BDA, induced nutritional contents in tomato and protected it against Alternaria alternata. The current study is the first report on the bioactivity of BDA and benzimidazole concerning the nutritional enhancement and plant defense improvement. (c) 2019 Society of Chemical Industry